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| 重组人补体受体1型SCR15-18片段对肠缺血再灌注损伤的保护作用 | |
| 引用本文： | 汪晓艳,刘高科,何莉,汪正清. 重组人补体受体1型SCR15-18片段对肠缺血再灌注损伤的保护作用[J]. 中国病理生理杂志, 2011, 27(10): 1927-1930. DOI: 10.3969/j.issn.1000-4718.2011.10.014 |
| 作者姓名： | 汪晓艳 刘高科 何莉 汪正清 |
| 作者单位： | 1. 第三军医大学基础部微生物教研室,重庆 400038; 2. 重庆市结核病防治所,重庆 400050 |
| 基金项目： | 国家自然科学基金资助项目，重庆市自然科学基金资助项目 |
| 摘 要： | 目的: 探讨人补体受体1型功能域SCR15-18 (CR1-SCR15-18)对大鼠肠缺血再灌注损伤的保护作用。方法: SD大鼠随机分为假手术组(SO)、缺血再灌注组(I/R)和CR1-SCR15-18保护组(CR1)。结扎肠系膜上动脉30 min,再灌注60 min,建立小肠缺血再灌注模型,再灌注前5 min注射CR1-SCR15-18蛋白(30 mg/kg)。测定肠黏膜血管的通透性、组织髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量;HE染色观察小肠病理改变并根据Chiu's方法评分;免疫组织化学法检测补体C3组分的沉积。结果: 与SO组相比,I/R组通透性增加,MPO活性和MDA含量显著升高,而SOD活性降低,肠黏膜病理损伤严重且有大量补体C3组分沉积。与I/R组相比,CR1组肠黏膜血管通透性显著降低,MPO活性和MDA含量显著降低,而SOD活性升高,肠黏膜损伤明显减轻,只有少量补体C3组分沉积。结论: CR1-SCR15-18蛋白抑制肠缺血再灌注时补体的活化,减轻肠组织损伤。 |
| 关 键 词： | 补体 补体受体1型 小肠 再灌注损伤 |
| 收稿时间： | 2011-04-02 |
Protective effect of recombinant SCR15-18 domain of human complement receptor type 1 on intestinal ischemia and reperfusion injury |
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| WANG Xiao-yan,LIU Gao-ke,HE Li,WANG Zheng-qing. Protective effect of recombinant SCR15-18 domain of human complement receptor type 1 on intestinal ischemia and reperfusion injury[J]. Chinese Journal of Pathophysiology, 2011, 27(10): 1927-1930. DOI: 10.3969/j.issn.1000-4718.2011.10.014 | |
| Authors: | WANG Xiao-yan LIU Gao-ke HE Li WANG Zheng-qing |
| Affiliation: | 1. Department of Microbiology, Third Military Medical University, Chongqing 400038, China; 2. Chongqing Tuberculosis Prophylactico-Therapeutic Institution, Chongqing 400050, China |
| Abstract: | AIM:To investigate the protective effect of recombinant SCR15-18 domain of human complement receptor type 1(CR1-SCR-15-18) on intestinal ischemia and reperfusion in a rat model.METHODS: Sprague-Dawley rats were randomly divided into 3 groups: sham operation(SO) group,ischemia and reperfusion(I/R) group and CR1-SCR15-18 treatment group.The superior mesenteric artery of the rats was clamped for 30 min followed by 60 min of reperfusion.PBS alone or CR1-SCR15-18 protein(30 mg/kg) in PBS was intravenously admini... |
| Keywords: | Complement Complement receptor type 1 Intestine Reperfusion injury |
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