凋亡相关基因Fas、bax和bcl—2蛋白在食管癌中的表达及其意义 |
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引用本文: | 张明,赵志毅,涂响安,刘丽萍,左连富.凋亡相关基因Fas、bax和bcl—2蛋白在食管癌中的表达及其意义[J].实用癌症杂志,2001,16(2):156-158. |
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作者姓名: | 张明 赵志毅 涂响安 刘丽萍 左连富 |
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作者单位: | 1. 526021,肇庆市第一人民医院外科 2. 050011,河北省肿瘤研究所 |
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摘 要: | 目的研究凋亡相关基因Fas、bax和bcl-2蛋白在食管癌发生发展中的作用。方法应用流式细胞术和细胞免疫荧光技术对70例食管鳞癌组织中Fas、bax和bcl-2蛋白进行定量检测。结果Fas阳性42例(60.0%),Fas蛋白表达与肿瘤的病理分级明显相关(P<0.05),与肿瘤临床分期和淋巴结转移与否无明显相关性(P>0.05);bax阳性39例(55.7%),bxl-2阳性65例(91.5%),bax和bxl-2蛋白表达与肿瘤的病理分级,临床分期和淋巴结转移无明显相关性(P>0.05)。结论Fas、bax和bcl-2基因与食管癌发生和发展有关。
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关 键 词: | 食管肿瘤 Fas蛋白 bax蛋白 bcl-2蛋白 流式细胞术 |
文章编号: | 1001-5930(2001)02-0156-03 |
修稿时间: | 2000年10月16 |
Expression and Significance of Fas、bax and bcl-2 in Esophageal Carcioma |
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Abstract: | Objective To study the role of Fas,bax and bcl 2 in occurrence and progression of esophageal carcioma.Methods The Fas,bax and bcl 2 protein expression were quantitantively determined by flow cytometry and celluar immunofluoresence technique in paraffin embeded tissues from 70 cases of esophageal squamous cell carcinoma.Results The positive expression rate of Fas protein was 60.0%(42/70),correlated with tumor grade( P <0.05) and not with tumor stage and lymph node metastasis( P > 0.05 ).The expression rate of bax and bcl 2 were 55.7%(39/70) and 91.5%(65/70) respectively,not correlated with tumor grade and stage and lymph node metastasis( P> 0.05).Conclusion Fas,bax and bcl 2 take part in the occurrence and progression of esophageal carcinoma. |
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Keywords: | Esophageal neoplasms Fas protein bax protein bcl 2 protein Flow cytometry |
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