应用细胞周期阻断法分析初诊白血病患者骨髓及外周血微核率

目的：对初诊白血病患者骨髓及外周血进行微核分析。方法：应用细胞周期阻断法对54例初诊白血病患者骨髓及外周血与30例健康人外周血淋巴细胞进行微核检测。结果：54例初诊白血病患者骨髓及外周血淋巴细胞微核率（MNR）和微核细胞率（MCR）,与30例健康人外周血相比,P〈0.01。同一患者骨髓与外周血淋巴细胞MNR和MCR相比,P〉0.05。急性淋巴细胞白血病（ALL）、急性髓细胞白血病（AML）与慢性粒细胞白血病（CML）患者骨髓及外周血淋巴细胞MNR和MCR相比,P〈0.05。结论：白血病患者发病初期即存在染色体不稳定现象,不同类型白血病患者微核检测结果间有差异,推测与白血病的发病机制密切相关。

Micronucleus analysis of bone marrow and peripheral blood by cytokinesis-blocked micronucleus test in preliminary-diagnosed leukemia patients

Objective：To analyze the micronucleus rate of bone marrow and peripheral blood in preliminary-diagnosed leukemia patients.Methods：The micronucleus of bone marrow and peripheral blood were detected by the cytokinesis-blocked micronucleus test in 54 preliminary-diagnosed leukemia patients,and those of peripheral blood were determined in 30 healthy individuals.Results：The micronuclear rate（MNR）and micronucleated cell rate（MCR）of peripheral blood in leukemia patients were significantly higher than those in healthy individuals（P0.01）.In leukemia patients,there was no significant difference in MNR and MCR in bone marrow and peripheral blood（P0.05）.The MNR and MCR of bone marrow and peripheral blood in chronic myeloid leukemia patients were significantly higher than those in acute lymphoblastic leukemia and acute myelocytic leukemia patients（P0.05）.Conclusion：Leukemia patients have chromosomal instability in the early period.The micronucleus rates are different between different types of leukemia,which may be related to the pathogenesis of leukemia.