| 马兰多糖的含量测定 |
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| 引用本文： | 王国凯,刘劲松,朱莉莉,王 刚.马兰多糖的含量测定[J].安徽中医学院学报,2013,32(6):84-85. |
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| 作者姓名： | 王国凯 刘劲松 朱莉莉 王 刚 |
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| 作者单位： | 安徽中医药大学药学院 现代中药安徽省重点实验室，安徽 合肥 230031 |
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| 基金项目： | 国家自然科学基金项目（81303220）；安徽省自然科学基金项目（11040606W220）；康缘中医药科技创新基金项目（KYCX 201003） |
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| 摘 要： | 目的 测定马兰中多糖含量,为马兰的应用和质量评价提供科学依据.方法 以葡萄糖为对照品,采用苯酚-硫酸比色法,在490 nm波长下测定多糖含量.结果 不同产地、采收时间的马兰多糖含量差异显著.结论 苯酚-硫酸比色法操作简单,重复性好,结果准确可靠,可用于马兰中多糖的含量测定.
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| 关 键 词： | 马兰 多糖 苯酚-硫酸比色法 |
Content Determination of Polysaccharides in Kalimeris indica(Linn.) Sch. |
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| WANG Guo-kai,LIU Jin-song,ZHU Li-li,WANG Gang.Content Determination of Polysaccharides in Kalimeris indica(Linn.) Sch.[J].Journal of Anhui Traditional Chinese Medical College,2013,32(6):84-85. |
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| Authors: | WANG Guo-kai LIU Jin-song ZHU Li-li WANG Gang |
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| Institution: | School of Pharmacy, Anhui University of Chinese Medicine & Anhui Key Laboratory of Modern Chinese Materia Medica, Anhui Hefei 230031, China |
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| Abstract: | Objective To determine the content of polysaccharides in Kalimeris indica(Linn.) Sch. from different areas and to provide a scientific basis for the application and quality evaluation of Kalimeris indica(Linn.) Sch. Methods With glucose as a control, the content of polysaccharides in Kalimeris indica(Linn.) Sch. from different areas was determined by phenol-sulfuric acid colorimetry at a wavelength of 490 nm. Results The content of polysaccharides varied significantly in Kalimeris indica(Linn.) Sch. produced in different areas and harvested at different times. Conclusion This determination method has the advantages of easy operation, good repeatability, and accurate results and is suitable for content determination of polysaccharides in Kalimeris indica(Linn.) Sch. from different areas. |
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| Keywords: | Kalimeris indica(Linn ) Sch Polysaccharides Phenol-sulfuric acid colorimetry |
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