紫皮石斛的质量标准研究

为建立云南省龙陵县紫皮石斛的地方质量标准，采用硫酸-苯酚法测定紫皮石斛中多糖的含量；采用高效液相色谱法测定药材中甘露糖的含量，并根据2010年版《中国药典》相关附录测定浸出物。结果表明紫皮石斛的显微鉴别特征性强；薄层色谱分离度好，斑点清晰；紫皮石斛含多糖质量分数为35.7%~52.1%（平均42.7%），甘露糖为27.8%~46.1%（平均35.8%），浸出物为4.5%~10.6%（平均7.38%）。所建方法操作简单，准确可靠，重复性好，制定的标准限度合理，可用于评价云南省龙陵县紫皮石斛的质量。

Studies on quality evaluation of Dendrobii devoniani

To establish the local quality standard for Dendrobii devoniani caulis from Longling, Yunnan, the pharmacognostic characteristics microscopic characteristics and TLC identification were developed. Sulfuric acid-phenol method was used to determine the content of polysaccharide.An HPLC method was adopted to determine the content of mannose, and extractives were determined according to the procedures recorded in the Appendix of Chinese Pharmacopoeia(2010). The results showed a strong characteristics microscopic of Dendrobii devoniani caulis, and its TLC identification had a good resolution with clear spots; the content of polysaccharide is 35.7%-52.1%(average 42.7%), mannose 27.8%-46.1%(average 35.8%), and extract 4.5%-10.6%(average 7.38%). The method is simple, accurate and reliable, with good reproducibility. The established standard is acceptable for quality evaluation of Dendrobii devoniani caulis from Longling, Yunnan.