| 用T7噬菌体展示技术筛选HCV核心蛋白的相互作用蛋白 |
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| 引用本文： | 黄英,蔡雪飞,张君,黄爱龙.用T7噬菌体展示技术筛选HCV核心蛋白的相互作用蛋白[J].第三军医大学学报,2007,29(10):876-878. |
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| 作者姓名： | 黄英 蔡雪飞 张君 黄爱龙 |
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| 作者单位： | 1. 重庆医科大学,附属第二医院感染科,重庆,400016
2. 重庆医科大学,感染性疾病分子生物学教育部省部共建重点实验室,重庆,400016 |
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| 摘 要： | 目的 用T7噬菌体筛选系统筛选HCV核心蛋白的相互作用蛋白.方法 应用T7噬菌体展示技术,以通过原核表达的方式得到HCV的核心蛋白作为靶分子,对噬菌体人肝细胞cDNA文库进行筛选,对筛选到的克隆进行DNA序列分析及同源性搜索.结果 经鉴定得到1个阳性克隆,确定了能与HCV核心蛋白相互作用的蛋白为:Smad interacting-protein 1(SIP1).结论 T7噬菌体展示筛选系统是筛选相互作用蛋白的一种简单、快速和有效的手段,筛选到的相互作用蛋白为进一步探讨核心蛋白的致病、致癌机制提供重要依据.
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| 关 键 词： | T7噬菌体展示技术 HCV核心蛋白 相互作用蛋白 |
| 文章编号： | 1000-5404（2007）10-0876-03 |
| 修稿时间： | 2006-09-20 |
Screening of proteins interacting with hepatitis C virus core protein from T7-phage display library |
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| HUANG Ying,CAI Xue-fei,ZHANG Jun,HUANG Ai-long.Screening of proteins interacting with hepatitis C virus core protein from T7-phage display library[J].Acta Academiae Medicinae Militaris Tertiae,2007,29(10):876-878. |
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| Authors: | HUANG Ying CAI Xue-fei ZHANG Jun HUANG Ai-long |
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| Institution: | 1 Department of Infectious Diseases, The Second Affiliated Hospital,2.Key Laboratory of Molecular Biology on Infection Diseases of Ministry of Education, Chongqing Medical University, Chongqing 400016, China |
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| Abstract: | Objective To screen and identify proteins that interact with the hepatitis C virus core protein by means of T7-phage display system. Methods The hepatitis C virus core protein was expressed by prokaryotic expression and used as selected molecule to biopan the T7 human liver cDNA library. The selected positive clones were identified by DNA sequence and analyzed with BLAST program in GenBank. Results After BLAST in all positive clones, one protein--Smad interacting protein 1 (SIP1) was found to interact with the hepatitis C virus core protein. Conclusion T7-phage display system is a convenient, rapid and effective method for screening interacting proteins. |
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| Keywords: | T7-phage display hepatitis C virus core protein interacting proteins |
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