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道地药材阳春砂不同居群的RAPD分析
引用本文:徐吉银,丁平. 道地药材阳春砂不同居群的RAPD分析[J]. 中药新药与临床药理, 2005, 16(3): 194-196
作者姓名:徐吉银  丁平
作者单位:广州中医药大学中药学院,广州,510405
摘    要:目的从 DNA分子水平上探讨阳春砂药材的道地性.方法对来自不同居群(春湾、蟠龙和云南 3个居群)的 49个阳春砂仁样品进行 RAPD分析,得到的条带用 Popgene 3.2和 Phylip软件进行数据处理.结果得出 3个居群阳春砂的聚合树状图,表明春湾和蟠龙两个居群的亲缘关系较近,云南居群与春湾、蟠龙两居群的亲缘关系较远.结论为从分子水平上探讨道地药材的形成提供客观依据.

关 键 词:阳春砂  道地性
文章编号:1003-9783(2005)03-0194-03
修稿时间:2005-02-14

Analysis on Genuineness of Amomum Villosum Lour. from Different Habitats by RAPD
XU Jiyin,DING Ping. Analysis on Genuineness of Amomum Villosum Lour. from Different Habitats by RAPD[J]. Traditional Chinese Drug Research & Clinical Pharmacology, 2005, 16(3): 194-196
Authors:XU Jiyin  DING Ping
Abstract:Objective To investigate the genuineness of Amomum Villosum Lour.at DNA molecular level.Methods Forty -nine samples from 3different habitats(Chunwan,Panlong,Yunnan)were analyzed by RAPD.The data of amp li-fied bands were analyzed by the softw are Popgene 3.2and Phylip.Results The molecular phylogenetic tree of Amomum Villosum Lour.from 3habitats indic ated that the Chunwan' s had a close consanguinity with the Panlong' s and the consan-guinity of the Yunnan' s was distant to the Chunwan' s and the Panlong' s.Conclusion This provide evidence of the gen-uineness of A.Villosum Lour.at molecular level.
Keywords:RAPD
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