| 斑马鱼uba6和uba7突变体的构建与鉴定 |
| |
| 引用本文： | 丁慧美,阮华,黄红辉.斑马鱼uba6和uba7突变体的构建与鉴定[J].中国比较医学杂志,2015,25(10):7-10. |
| |
| 作者姓名： | 丁慧美 阮华 黄红辉 |
| |
| 作者单位： | 淡水鱼类资源与生殖发育教育部重点实验室, 三峡库区生态环境与生物资源省部共建 国家重点实验室培育基地, 西南大学生命科学学院, 重庆 400715;淡水鱼类资源与生殖发育教育部重点实验室, 三峡库区生态环境与生物资源省部共建 国家重点实验室培育基地, 西南大学生命科学学院, 重庆 400715;淡水鱼类资源与生殖发育教育部重点实验室, 三峡库区生态环境与生物资源省部共建 国家重点实验室培育基地, 西南大学生命科学学院, 重庆 400715 |
| |
| 基金项目： | 国家自然科学基金面上项目(31071286);西南大学中央高校基本科研业务费(XDJK2014A013;2362014xk02)。 |
| |
| 摘 要： | 目的 构建斑马鱼uba6和uba7突变体。方法 运用TALEN技术在斑马鱼uba6和uba7基因编码区引进突变,并测序鉴定突变类型。结果 成功获得了两种突变类型的uba6和三种突变类型的uba7斑马鱼突变体。结论 成功构建了斑马鱼uba6和uba7突变体,为进一步研究斑马鱼uba6和uba7基因的功能提供了材料。
|
| 关 键 词： | 斑马鱼 uba6基因 uba7基因 TALEN |
| 收稿时间： | 2015/6/25 0:00:00 |
| 修稿时间： | 2015/8/18 0:00:00 |
Construction and identification of zebrafish uba6 and uba7 mutants by TALEN |
| |
| Ding Huimei,Ruan Hua and Huang Honghui.Construction and identification of zebrafish uba6 and uba7 mutants by TALEN[J].Chinese Journal of Comparative Medicine,2015,25(10):7-10. |
| |
| Authors: | Ding Huimei Ruan Hua and Huang Honghui |
| |
| Institution: | Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, State Key Laboratory Breeding Base of Eco-Environments and Bio-Resources of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China;Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, State Key Laboratory Breeding Base of Eco-Environments and Bio-Resources of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China;Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, State Key Laboratory Breeding Base of Eco-Environments and Bio-Resources of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China |
| |
| Abstract: | Objective To construct zebrafish uba6 and uba7 mutants. Method In-dels were introduced into coding regions of zebrafish uba6 and uba7 gene by TALEN technology, and mutation types were identified by sequencing. Results We obtained two uba6 and three uba7 mutants with different small fragment deletions resulting in reading frame shifts. Conclusion These uba6 and uba7 mutants provided starting materials for the study of zebrafish uba6 and uba7 gene functions. |
| |
| Keywords: | Zebrafish uba6 uba7 TALEN |
|
| 点击此处可从《中国比较医学杂志》浏览原始摘要信息 |
| 点击此处可从《中国比较医学杂志》下载免费的PDF全文 |
|
