| 单抗C225致人肺鳞癌H-520细胞生长抑制和凋亡增加的研究 |
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| 引用本文： | 王壮杰,张颖东,赵海鹰,赵勇.单抗C225致人肺鳞癌H-520细胞生长抑制和凋亡增加的研究[J].现代肿瘤医学,2010,18(9). |
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| 作者姓名： | 王壮杰 张颖东 赵海鹰 赵勇 |
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| 作者单位： | 1. 中国人民解放军第255医院肿瘤诊疗中心,河北,唐山,063000
2. 中国科学院动物研究所生物膜国家重点实验室,北京,100101 |
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| 摘 要： | 目的:观察表皮生长因子受体单克隆抗体C225对人肺鳞癌细胞系(H-520)生长、凋亡和周期分布的影响.方法:流式细胞检测H-520细胞EGFR表达比例,将鼠抗人EGFR一抗和FITC标记的羊抗鼠二抗加入细胞悬液中,孵育、漂洗重悬细胞后流式检测.MTY法测定C225抑制H-520细胞生长最佳浓度和最佳时间,将不同浓度C225加入指数生长的细胞培养液中,继续培养一定时间(48h),检测细胞生长抑制率.流式细胞仪检测细胞凋亡以及细胞周期.结果:H-520细胞中EGFR表达比例高达82.25%.C225抑制H-520细胞生长的最佳浓度是40nmol/L,最佳作用时间是72h.细胞凋亡实验结果显示,H-520细胞自然凋亡率为5.56%±0.62%,C225可使其凋亡百分率上升到13.75%±0.83%(P＜0.05).细胞周期分布显示40nmol/L C225可使细胞阻滞于G0+G1期,S期细胞比例下降.结论:C225对H-520细胞生长抑制作用,可能与细胞G0+G1期阻滞后凋亡有关.
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| 关 键 词： | H-520细胞系 肿瘤 |
The radiosensitizing effect of C225 on human non -small cell lung cancer cell line H -520 |
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| WANG Zhuang-jie,ZHANG Ying-dong,ZHAO Hai-ying,ZHAO Yong.The radiosensitizing effect of C225 on human non -small cell lung cancer cell line H -520[J].Journal of Modern Oncology,2010,18(9). |
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| Authors: | WANG Zhuang-jie ZHANG Ying-dong ZHAO Hai-ying ZHAO Yong |
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| Institution: | WANG Zhuang-jie1,ZHANG Ying-dong1,ZHAO Hai-ying1,ZHAO Yong2 1Cancer Center,Chinese People' s Liberation Army No.255 Hospital,Tangshan 063000,China,2 Transplantation Biology Research Division,State Key Laboratory of Biomembrane and Membrane Biotechnology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China. |
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| Abstract: | Objective:To investigate the effects of C225 (cetuximab),a chimeric human-mouse anti-epithelial growth factor receptor monoclonal antibody,on the growth,apoptosis and cell cycle of human non-small cell lung cancer cell line H-520 and explore whether C225 treatment is appropriate for non-small cell lung cancer patients in clinics.Methods:With flow cytometric analysis,we first detected the expression level of EGFR on H-520 cells.To determine the appropriate concentration and time for C225 treatment,we treated... |
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| Keywords: | C225 |
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