肝门部胆管癌细胞系FRH 0201与正常肝细胞系L02蛋白质组学差异分析

目的：分析肝门部胆管癌细胞系FRH0201与正常肝细胞系L02的蛋白质表达差异,筛选肝门部胆管癌的潜在分子标志物。方法：应用表面增强激光解吸离子化（surface enhanced laser desorption/ionization,SELDI）蛋白质芯片技术检测肝门部胆管癌及正常肝细胞系的蛋白质谱。用PBSII-C型蛋白质芯片阅读机读取数据,采用Proteinchip软件分析数据。结果：IMAC3、WCX2两种蛋白芯片共捕获144个蛋白峰,发现16个差异蛋白。与正常肝细胞系L02蛋白谱相比,4个蛋白在肝门部胆管癌细胞系FRH0201中高表达,12个蛋白在肝门部胆管癌细胞系FRH0201中低表达。结论：肝门部胆管癌与正常肝细胞存在差异蛋白表达,这些差异蛋白有可能成为潜在的肝门部胆管癌标志物,对其进行研究有助于了解肝门部胆管癌的发病机制。

Analysis of differential expression of proteins in portal cholangiocarcinoma cell line FRH 0201 and hepatocellular cell line L02

OBJECTIVE:To analyze the differential expression of proteins in portal cholangiocellular carcinoma cell line FRH 0201 and normal hepatocellular cell line L02,and to screen potential molecular markers for diagnosis of hilar cholangiocarcinoma. METHODS: Surface enhanced laser desorption/ionization (SELDI) mass spectrometry with Proteinchip IMAC3 and WCX2 was performed to compare the expressed proteins in portal cholangiocellular carcinoma cell line FRH 0201 and hepatocellular cell line L02. Protein profiling was examined by PBSII-C Protein Chip Reader and the proteome database was analyzed by Proteinchip Software 3.0.2. RESULTS: 144 protein peaks in FRH 0201 and L02 cell lines were captured by Proteinchip IMAC3 and WCX2,16 differential proteins were found. Four proteins were up-regulated and 12 proteins down-regulated in portal cholangiocellular carcinoma cell line FRH 0201 as compared with L02 cell line. CONCLUSION: The differential proteins could be candidate biomarkers of portal cholangiocarcinoma. The study may contribute to understanding the mechanisms of development of portal cholangiocarcinoma