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短果茴芹抗氧化活性成分的提取及筛选
引用本文:卢静,钱文辉,关爽,邓旭明,宋晓飞,刘昕阳,王大成.短果茴芹抗氧化活性成分的提取及筛选[J].职业与健康,2011,27(9):967-969.
作者姓名:卢静  钱文辉  关爽  邓旭明  宋晓飞  刘昕阳  王大成
作者单位:1. 吉林大学军需科技学院食品质最与安全系,长春市,130062;吉林大学畜牧兽医学院生物技术系
2. 吉林大学军需科技学院食品质最与安全系,长春市,130062
3. 吉林大学畜牧兽医学院生物技术系
摘    要:目的对短果茴芹抗氧化活性成分进行提取,并了解其提取物的抗氧化效果。方法采用邻苯三酚自氧化体系、Fenton反应体系和H2O2诱导的氧化损伤模型,了解短果茴芹4种提取物的抗氧化作用。结果所获得的4种提取物均具有抗氧化作用,其中乙酸乙酯层提取物作用最强,这与乙酸乙酯层提取物具有较强的清除O.-2和.OH能力有关;石油醚层提取物虽然也具有较强的清除O.-2和.OH能力,但对人肝癌细胞BEL-7404的氧化损伤无保护作用,反而有抑制肿瘤的效果。结论短果茴芹富含抗氧化活性成分,值得深入研发。

关 键 词:短果茴芹  提取物  抗氧化  O.-2清除率  .OH清除率  H2O2损伤

Extraction and Isolation of Antioxidant Components from Pimpinella Brachycarpa
LU Jing,QIAN Wen-hui,GUAN Shuang,DENG Xu-ming,SONG Xiao-fei,LIU Xin-yang,WANG Da-cheng.Extraction and Isolation of Antioxidant Components from Pimpinella Brachycarpa[J].Occupation and Health,2011,27(9):967-969.
Authors:LU Jing  QIAN Wen-hui  GUAN Shuang  DENG Xu-ming  SONG Xiao-fei  LIU Xin-yang  WANG Da-cheng
Institution:LU Jing,QIAN Wen-hui,GUAN Shuang,DENG Xu-ming,SONG Xiao-fei,LIU Xin-yang,WANG Da-cheng(College of Quartermaster Technology,Jilin University,Changchun,130062,China)
Abstract:Objective]To extract antioxidant components from pimpinella brachycarpa,and study the antioxidant effect of its extraction.Methods]Pyrogallol autoxidation system,fenton reaction system and H2O2-induced oxidative injury model were adopted to study the antioxidant effect of 4 kinds of Pimpinella brachycarpa's extractsResults]All of the 4 extracts had antioxidant capacity,especially the extract obtained from ethyl acetate.It related with the ethyl acetate extract's strong capability of eliminating O-·2and·OH.Through petroleum ether extracts had strong capability of eliminating O-·2and·OH,it had no sheltering effect against oxidative damage of BEL-7404 human liver cancer cell,achieving the goals of tumor inhibition.Conclusion]Pimpinella brachycarpa is rich in antioxidant substance and deserving deep investigation.
Keywords:Pimpinella brachycarpa  Extract  Anti-oxidation  O-·2Scavenging Activities  ·OH Scavenging Activities  H2O2 induced Oxidative stress
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