镍接触者尿中镍的石墨炉原子吸收光谱测定法

目的建立尿中镍的石墨炉原子吸收光谱法测定职业镍接触者的尿镍水平,并与非职业镍接触者尿镍进行比较。方法用石墨炉原子吸收光谱法直接进样测定职业和非职业镍接触者尿中镍浓度,然后进行统计分析。结果该方法在2～100μg/L范围内有良好的线性关系,r=0.999 9,检出线为1.3μg/L,回收率93.9%～106.2%,RDS2.4%～5.5%。职业镍接触者尿镍浓度高于非职业接触者,经统计分析,两者的差异具有统计学意义(P<0.01)。结论该方法可作为监测职业镍接触者体内镍摄入水平。

Determination of Nickel Level in Urine Specimens of Workers with Occupational Exposure by GFAAS

[Objective]To establish the method for detection of nickel level in urine of workers with occupational exposure by Graphite furnace atomic absorption spectrometry（GFAAS）,and to compare the results with workers without occupational exposure.[Methods]GFAAS was used to determine the nickel level in urine of workers with and without exposure.Then the results were analyzed statistically.[Results]Good linear relationship was showed in the range of 2～100 μg/L,r=0.999 9,detection limit was 13μg/L,recoveries were 93.9%～106.2%,and RSD was 2.4%～5.5%.Nickel level in urine of workers with exposure was higher than that of workers without exposure,the difference was significant（P〈0.01）.[Conclusion]This method can be used to monitor the nickel intake level in vivo of workers with occupational exposure.