甲醛对V79细胞DNA损伤的实验研究

目的探讨甲醛（FA）的DNA损伤机制。方法应用单细胞凝胶电泳技术研究不同浓度FA对V79细胞（中国仓鼠肺成纤维细胞）的DNA损伤程度,以及剂量依赖关系。结果单细胞凝胶电泳结果显示,在不同浓度FA（100～1 600μmol/L）彗星细胞拖尾率、尾长及头/尾比随着浓度的增加而减少,且差异具有统计学意义（P〈0.01）;特别是在1 600μmol/L时在图片中未见有彗星细胞出现,其显示的DNA损伤类型是DNA-蛋白质交联;但是FA的浓度与拖尾细胞率、尾长及头/尾比之间不存在线性关系。结论该研究结果认为,在体外培养条件下,一定浓度的FA能够引起细胞DNA损伤,主要表现为DNA-蛋白质交联。

Experimental Study on DNA of V79 Cell Damaged by Formaldehyde

[Objective]Discussion on FA＇s DNA damage.[Methods]SCGE（single cell gel electrophoresis） was used to investigate the degree of V79（Chinese hamster lung fibroblasts）cells＇ DNA damage and dose-dependent relationship between the toxic concentration and V79 cell＇s DNA damage.[Results]Different concentrations FA（100～ 1 600 μmol / L） resulted in the comet cell tail ratio,tail length and head / tail ratio increased.with the concentration decreased,and there was statistically significant（P 0.01）.particularly in the 1 600 μmol / L group,there was no comet cells.However,there was no any the linear relationship between the concentration of FA and cells with the rate of tail,tail length and head / tail ratio.[Conclusion]In vitro conditions,a certain concentration of the FA can induced DNA damage,mainly DNA-protein crosslinks.