The effects of serotonin1A receptor on female mice body weight and food intake are associated with the differential expression of hypothalamic neuropeptides and the GABAA receptor |
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| Affiliation: | 1. Department of Biology, College of Staten Island, City University of New York, Staten Island, NY 10314, USA;2. Institute for Macromolecular Assemblies, City University of New York, Staten Island, NY 10314, USA;3. Department of Chemistry, College of Staten Island, City University of New York, Staten Island, NY 10314, USA;1. Department of Experimental and Clinical Physiology, Medical University of Warsaw, 02-106 Warsaw, Poland;2. Department of Pathophysiology, Immunology and Pathology, Institute of Rheumatology, Warsaw, Poland;1. Laboratory of Molecular and Cellular Screening Processes, Center of Biotechnology of Sfax, Tunisia;2. Department of Otorhinolaryngology, Habib Bourguiba Teaching Hospital, University of Sfax, Tunisia;3. Department of Ophthalmology, Habib Bourguiba Teaching Hospital, University of Sfax, Tunisia;4. Department of Endocrinology, Hedi Chaker Teaching Hospital, University of Sfax, Tunisia;5. The Jackson Laboratory, Bar Harbor, ME, USA;1. Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, USA;2. Department of Neurosciences, Case Western Reserve University, Cleveland, OH, USA;3. Florey Institute of Neuroscience and Mental Health, University of Melbourne, Melbourne, Australia;1. Dept. Animal Ecology, Evolution and Biodiversity, Ruhr University Bochum, Universitätsstr. 150, 447801 Bochum, Germany;2. Department of Animal Ecology I and BayCEER, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany;3. Department of Biology II, Ludwig-Maximilians-University Munich, Grosshaderner Str. 2, 82152 Planegg-Martinsried, Germany;1. Department of General Thoracic Surgery, Keio University School of Medicine, Tokyo, Japan;2. Department of General Thoracic Surgery, Kawasaki Municipal Hospital, Kanagawa, Japan;3. Department of Medicine, Saitama Medical University Hospital, Saitama, Japan;1. Department of Medicine, University of Colorado, Anschutz Medical Campus, Aurora, CO, USA;2. Barbara Davis Center for Childhood Diabetes, University of Colorado, Anschutz Medical Campus, Aurora, CO, USA;3. Department of Epidemiology, Colorado School of Public Health, University of Colorado, Anschutz Medical Campus, Aurora, CO, USA |
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| Abstract: | Both common eating disorders anorexia nervosa and bulimia nervosa are characteristically diseases of women. To characterize the role of the 5-HT1A receptor (5-HT1A-R) in these eating disorders in females, we investigated the effect of saline or 8-hydroxy-2-(di-n-propylamino) tetralin (8-OH-DPAT) treatment on feeding behavior and body weight in adult WT female mice and in adult 5-HT1A-R knockout (KO) female mice. Our results showed that KO female mice have lower food intake and body weight than WT female mice. Administration of 8-OH-DPAT decreased food intake but not body weight in WT female mice. Furthermore, qRT-PCR was employed to analyze the expression levels of neuropeptides, γ-aminobutyric acid A receptor subunit β (GABAA β subunits) and glutamic acid decarboxylase in the hypothalamic area. The results showed the difference in food intake between WT and KO mice was accompanied by differential expression of POMC, CART and GABAA β2, and the difference in body weight between WT and KO mice was associated with significantly different expression levels of CART and GABAA β2. As such, our data provide new insight into the role of 5-HT1A-R in both feeding behavior and the associated expression of neuropeptides and the GABAA receptor. |
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| Keywords: | Serotonin Neuropeptides Food intake Body weight Gene expression qRT-PCR |
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