Telencephalin protects PAJU cells from amyloid beta protein-induced apoptosis by activating the ezrin/radixin/moesin protein family/phosphatidylinositol-3-kinase/protein kinase B pathway |
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Authors: | Heping Yang Dapeng Wu Xiaojie Zhang Xiang Wang Yi Peng Zhiping Hu |
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Institution: | 1 Department of Neurology, Shangrao No. 5 People’s Hospital, the Second Affiliated Hospital of Nanchang University, Shangrao 334000,Jiangxi Province, China2 Department of Neurology, Ruikang Hospital Affiliated to Guangxi Traditional Chinese Medical University, Nanning 530011, Guangxi ZhuangAutonomous Region, China3 Department of Neurology, Xiangya Second Hospital, Central South University, Changsha 410011, Hunan Province, China4 Department of Psychiatry, Xiangya Second Hospital, Central South University, Changsha 410011, Hunan Province, China |
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Abstract: | Telencephalin is a neural glycoprotein that reduces apoptosis induced by amyloid beta protein in the human neural tumor cell line PAJU. In this study, we examined the role of the ezrin/radixin/moesin protein family/phosphatidylinositol-3-kinase/protein kinase B pathway in this process. Western blot analysis demonstrated that telencephalin, phosphorylated ezrin/radixin/moesin and phosphatidy-linositol-3-kinase/protein kinase B were not expressed in PAJU cells transfected with empty plasmid,while they were expressed in PAJU cells transfected with a telencephalin expression plasmid. After treatment with 1.0 nM amyloid beta protein 42, expression of telencephalin and phosphorylated phosphatidylinositol-3-kinase/protein kinase B in the transfected cells gradually diminished, while levels of phosphorylated ezrin/radixin/moesin increased. In addition, the high levels of telencephalin,phosphorylated ezrin/radixin/moesin and phosphatidylinositol-3-kinase/protein kinase B expression in PAJU cells transfected with a telencephalin expression plasmid could be suppressed by the phosphatidylinositol-3-kinase inhibitor LY294002. These findings indicate that telencephalin activates the ezrin/radixin/moesin family/phosphatidylinositol-3-kinase/protein kinase B pathway and protects PAJU cells from amyloid beta protein-induced apoptosis. |
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Keywords: | telencephalin/intercellular adhesion molecule 5 amyloid beta protein ezrin/radixin/moesin family proteins/phosphatidylinositol-3-kinase/protein kinase B signal transduction neural regeneration |
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