阵发性睡眠性血红蛋白尿症患者血清对CD34+细胞生长的影响

为探讨阵发性睡眠性血红蛋白尿症（PNH）患血清对造血干／祖细胞增殖的影响，应用单个细胞培养及液体、半固体群体细胞培养方法，将病人血清与正常人血清分别加入培养体系中，观察不同血清对正常人及病人正常及异常表型的CD34^ 细胞的直接影响。发现在单个细胞培养条件下，加入PNH血清与加入正常人血清时，正常人及PNH两种表型的CD34^ 细胞在细胞分裂、集落形成、较大集落形成的比例、单个细胞扩增能力及细胞总数诸方面均无明显差别（P＞0．05）。在半固体集落培养时，加入病人或正常人血清，对正常人及病人正常表型CD34^ 细胞的集落形成能力的影响无显差异（P＞0．05），而病人CD34^ CD59^-造血干／祖细胞的集落形成率在加入病人血清组高于加入正常人血清组（P＜0．05）。结果说明，在单个细胞及群体细胞液体培养条件下，加入病人血清与加入正常人血清，对正常人以及病人正常或异常表型造血干／祖细胞和长的影响无显差别，而在半固体培养条件下，则显示病人血清更有利于患的异常表型CD34^ 细胞的集落生成。

The Effects of Sera From Patients with Paroxysmal Nocturnal Hematoglobinuria on the Growth of CD34 + Cells

To investigate the effects of sera on the growth of single CD34 + cells from patients with paroxysmal nocturnal hematoglobinuria(PNH), sera from both PNH patients and normal individuals were added separately to the single cell culture system and semi solid colony formation system. The growth of the normal CD34 + and PNH CD34 +CD59 + and CD34 +CD59 - cells was evaluated. No growth difference was found for growth of the normal CD34 + and PNH CD34 +CD59 + cells when PNH or normal sera were added to the culture media in either single cell culture or colony formation culture. While no difference was detected for single PNH CD34 +CD59 - cells growth when PNH or normal sera were added, more colonies were observed in semi solid culture with PNH sera. A conclusion was reached that compared with those from normal controls, the sera from PNH patients had no significant influence on single hematopoietic stem/progenitor cells derived from normal subjects and from PNH patients, but the PNH sera might promote the colony formation of the CD34 +CD59 - cells in semi solid culture.