三羟异黄酮对小鼠辐射损伤后造血功能的影响

目的 探讨三羟异黄酮(genistein, GEN)对辐射损伤造血功能的影响.方法 6.0 Gy γ射线一次全身照射BALB/c雄性小鼠复制出造血损伤模型.照射前24 h给予GEN灌胃(160 mg/kg体质量),分别观察照射后1、3、7、14、21、30 d不同时相点小鼠血清对粒单系集落形成单位(colony-forming unit-granulocyte/macrophage, CFU-GM)和红系集落形成单位(colony forming unit-erythroid, CFU-E)的支持或抑制活性.结果 辐射前24 h给予GEN,辐射后该组小鼠血清造血刺激活性显著增加,使正常小鼠骨髓有核细胞CFU-GM、CFU-E形成数量明显增多,而且对CFU-GM的刺激活性较CFU-E强.同时,该组小鼠照后血清对CFU-GM、CFU-E的抑制活性明显降低,但对CFU-E和CFU-GM的抑制活性差异不明显.结论 提高辐射损伤后小鼠血清刺激活性可能是GEN防护放射性造血损伤、促进受损造血系统重建的分子机制之一.

Effect of genistein on hematopoiesis in irradiated mice

Objective To study the effect of genistein on the hematopoiesis in irradiated mice. Methods Adult male BALB/c mice were administered orally with genistein (160 mg/kg) 24 h before whole body exposure to gamma irradiation at a sublethal dose of 6.0 Gy. On day 1, 3, 7, 14, 21 and 30 after irradiation injury, the hematopoiesis-stimulating and hematopoiesis-suppressing activities of the serum were determined by CFU-E and CFU-GM culture in vitro, respectively. Results The hematopoiesis-stimulating activities of serum enhanced distinctly in all irradiated groups. With genistein administration 24 h before irradiation, the hematopoiesis-stimulating activities of serum became enhanced, resulting in the increase of numbers of CFU-GM and CFU-E, as compared to those in only irradiated mice, and stimulating activities on CFU-GM were more enhanced than that on CFU-E. The hematopoiesis-suppressing activities of serum were decreased in genistein pre-treated mice, but showed no significant difference between CFU-GM and CFU-E. Conclusion Enhancing hematopoiesis-stimulating activity of serum in the irradiated mice may be one of the important mechanisms of genistein against irradiation-induced hematopoietic syndrome and accelerating restoration of hematopoiesis.