骨关节退行性病变临床数据和样本资源库的建立及管理

目的利用北京积水潭医院骨科医疗优势，建立骨关节退行性病变临床数据和样本资源库，为进一步开展科学研究做好资源准备。方法建立标准化操作流程及管理体系，收集2007年7月以来因骨关节退行性病变而行手术切除的患者的组织及血液标本，组织标本从离体到冻存控制在10rain以内，且标本尽量多管分装，分别按不同要求处理后放入-80℃超低温冰箱冷冻保存，同时收集相应患者的临床信息，进行数据库信息化管理。结果从2007年7月至2011年11月共收集了2605例骨关节退行性病变患者的临床信息及标本，共15188管份组织与血标本。向科研及临床工作者提供临床信息及组织标本165份，血标本2005管份，以及相应患者的临床信息，分别应用于分子生物学、流行病学等分析研究，并有相关文章发表。结论骨关节退行性病变样本资源库的建立，是开展相关疾病研究的重要的基础性工作，规范的样本收集操作流程及完善的管理体系是资源库质量的重要保证。

Establishment and management of tissue biobank of osteoarthritis

Objective To establish tissue biobank of osteoarthritis in Beijing Jishuitan Hospital to promote orthopedic research study in China. Methods Fresh tissue or blood samples were collected from patients who underwent surgical operation for osteoarthritis since July 2007. Clinical information of the patients was also collected. Microsoft Access database system was used for the management of information. Results From July 2007 to November 2011, a total of 2605 medical records and 15 188 tissue or blood samples were collected. Among them, 165 tissue samples and 2005 blood samples were provided for molecular biology or epidemiological research. Conclusion Human tissue biobank is important for research work. Present osteoarthritis tissue collection and storage is feasible and could supply quality samples for study.