示波极谱法测定血清和组织中的亚硝酸盐

用示波极谱法测定血清和组织匀浆中的亚硝酸盐。取０．１０ｍｌ血清或组织匀浆，样液中的ＮＯ－２在稀盐酸介质中，与磺胺和盐酸萘乙二胺反应生成偶氮化合物，在氨碱性介质中，该偶氮化合物可产生灵敏的极谱波，波高与ＮＯ－２含量在一定范围内成正比关系。本法检出限为０．５６μｇ／Ｌ（相应血清浓度和组织含量分别为０．６１μｍｏｌ／Ｌ和０．２２μｇ／ｇ），相对标准差为６．１％～７．８％。加标回收率为９２．０％～１０８．０％（平均１００．６％）。本法具有灵敏度高，操作简便和样品不需除蛋白即可直接测定等优点。

Determination of Nitrite in Serum and Tissue by Oscillopolarography

A method for determining nitrite in serum and placenta tissue by oscillopolaropgraphy is reported in this paper.Nitrite in 0.10 ml of serum or placenta homogenate reacts with sulfanilmide and N1 (1Naphthyl)ethylendiamindihydrochloride in diluted hydrochloric acid medium to form an azo compound which can produce a sensitive reductive polarographic wave in ammonium alkaline medium. The wave height is directly proportional to the nitrite content. the method has a detection limit of 0.56 g/L (corresponding to the nitrite content of 0.61 mol/L in serum or 0.22 g/g), RSDs of 61%7.8% and recoveries of 92.08.0%(mean value 100.6%).The method is sensitive, simple and can determine the nitrite directly without removal of the proteins in the samples.