| VEGF反义寡核苷酸对C6胶质瘤细胞VEGF 表达抑制作用的定量和定性检测 |
| |
| 引用本文： | 李维方,张光霁,朱诚,金由辛,卢亦成. VEGF反义寡核苷酸对C6胶质瘤细胞VEGF 表达抑制作用的定量和定性检测[J]. 解放军医学杂志, 2002, 27(1): 32-33,I002 |
| |
| 作者姓名： | 李维方 张光霁 朱诚 金由辛 卢亦成 |
| |
| 作者单位： | 1. 第四军医大学长征医院,上海,200003
2. 中国科学院生物化学研究所 |
| |
| 基金项目： | 上海市优秀学科带头人资助计划基金资助课题(编号 99XD14002); |
| |
| 摘 要： | 为观察VEGF反义寡核苷酸对C6胶质瘤细胞VEGF表达抑制作用，应用免疫荧光流式细胞技术和免疫细胞化学定量和定性观察反义、正义、错义寡核苷酸对C6胶质瘤细胞VEGF表达的影响。结果显示，VEGF反义寡核苷酸对C6胶质瘤细胞VEGF的表达有明显抑制作用，而且抑制作用存在浓度依赖性。提示VEGF反义寡核苷酸能够有效地抑制C6胶质瘤细胞VEGF的表达。
|
| 关 键 词： | 内皮生长因子 反义寡核苷酸类 神经胶质瘤 |
INHIBITION OF VEGF EXPRESSION IN C6 GLIOMA CELLS WITH VEGF ANTISENSE OLIGONUCLEOTIDES |
| |
| Li Weifang,Zhang Guangji,Zhu Cheng et al. INHIBITION OF VEGF EXPRESSION IN C6 GLIOMA CELLS WITH VEGF ANTISENSE OLIGONUCLEOTIDES[J]. Medical Journal of Chinese People's Liberation Army, 2002, 27(1): 32-33,I002 |
| |
| Authors: | Li Weifang Zhang Guangji Zhu Cheng et al |
| |
| Affiliation: | Li Weifang,Zhang Guangji,Zhu Cheng et al . Changzheng Hospital,Second Military Medical University,Shanghai 200003 |
| |
| Abstract: | To observe the inhibition of vascular endothelial growth factor(VEGF) expression in C 6 glioma cells with VEGF antisense oligonucleotides.VEGF expression in C 6 glioma cells was examined quantitatively and qualitatively with immunofluorescence flow cytometric analysis and immunohistochemistry.Results showed that VEGF antisense ODNs inhibited the VEGF expression in C 6 glioma cells,and the inhibition was concentration dependent.This indicates that VEGF antisense ODNs can effectively suppress the VEGF expression in C 6 glioma cells. |
| |
| Keywords: | endothelial growth factor oligonucleotides antisense glioma |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
