RNAi-dependent and -independent antiviral phenotypes of chromosomally integrated shRNA clones: Role of VASP in respiratory syncytial virus growth |
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| Authors: | Alla Musiyenko Vira Bitko Sailen Barik |
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| Affiliation: | (1) Department of Biochemistry and Molecular Biology, College of Medicine, University of South Alabama, 307 University Blvd., Mobile, AL 36688-0002, USA |
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| Abstract: | Stable RNA interference (RNAi) is commonly achieved by recombinant expression of short hairpin RNA (shRNA). To generate virus-resistant cell lines, we cloned a shRNA cassette against the phosphoprotein gene of respiratory syncytial virus (RSV) into a polIII-driven plasmid vector. Analysis of individual stable transfectants showed a spectrum of RSV resistance correlating with the levels of shRNA expressed from different chromosomal locations. Interestingly, resistance in a minority of clones was due to mono-allelic disruption of the cellular gene for vasodilator-stimulated phosphoprotein (VASP). Thus, pure clones of chromosomally integrated DNA-directed RNAi can exhibit gene disruption phenotypes resembling but unrelated to RNAi. |
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| Keywords: | Chromosomal Antiviral RNAi Plasmid vector Mutation Infectious disease |
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