大鼠腰神经根受压后背根神经节及脊髓后角神经元型一氧化氮合酶阳性神经元的变化

目的探讨大鼠腰神经根受压后后背根神经节和脊髓后角神经元型一氧化氮合酶阳性神经元的变化规律.方法选用12只Wistar大鼠,随机分为实验组和正常组,采用免疫组织化学ABC法结合图像分析系统进行研究.结果大鼠腰神经根受压后4周,实验组背根神经节中神经元型一氧化氮合酶阳性神经元细胞数、平均面积明显增多,脊髓后角神经元型一氧化氮合酶阳性神经末梢也明显增多,与正常组相比均有显著性差异.神经元形态以中、小型细胞为主.结论大鼠腰神经根受压后感觉神经元神经元型一氧化氮合酶表达上调,提示神经元型一氧化氮合酶可能与根性腰腿痛的发生有关.

CHANGES OF NITRIC OXIDE SYNTHASE-POSITIVE NEURONS IN DORSAL ROOT GANGLIA AND DORSAL HORN FOLLOWING A CHRONIC CONSTRICTION TO THE RAT LUMBAR NERVE ROOT

Objective: To study the changes of nitric oxide synthase (NOS1) positive neurons in dorsal root ganglias (DRG) and dorsal horn following a chronic constriction to the rat lumbar nerve root. Mechods: Both experiment and control group consisted of 12 Wistar rats. Immunohistochemistry combined with image quantitative analysis were performed. Results: After a chronic constriction injury to the rat lumbar nerve root for 4 weeks, there was a statistically significant increase in quantity and average area of NOS positive neurons in DRG and in average area of NOS positive neurous endings in dorsal horn in the experiment group compared the control group; NOS positive neurons were middle and small neurons. Conclusion: The expression of NOS in dorsal root ganglions and dorsal horn of spinal cord is higher after a chronic constriction injury to the rat lumbar nerve root. NO takes part in radicular pain modulation.