p16基因对脑胶质瘤细胞生长及化疗敏感性的影响

目的：探讨ｐ１６基因在脑胶质瘤发生发展过程中的作用及其与脑胶质瘤细胞对鬼臼噻吩苷、顺铂化疗敏感性间的关系。方法：将外源野生型ｐ１６基因导入胶质瘤细胞株Ｕ２５１，观察ｐ１６基因长期稳定转染对胶质瘤细胞的作用，并筛选阳性克隆。同时以空载体质粒ＰＣＤＮＡ３为对照。Ｎｏｒｔｈｅｒｎ杂交检测ｐ１６基因表达，对转染后细胞生长情况、细胞周期、裸鼠致瘤能力的变化及细胞对鬼臼噻吩苷和顺铂敏感性的变化进行分析。结果：

Effects of p16 gene on chemosensitivity of human glioma cell line to teniposide and cisplatin

Purpose:To determine the effects on the cell growth,tumorigenicity and chemosensitivity of p16/CDK4I in human glioma. Methods:p16 gene was transfected into U251 cells by lipofectin. Expression of exogenous p16 gene was confirmed by Northern blot. The effects of exogenous p16 gene on the growth and chemosensitivity to teniposide and CDDP were examined. Results:Expression of exogenous p16 gene inhibited the growth of U251 dramatically in vivo and in vitro. G1 arrest of tumor cells was observed. However, wt p16 positive U251 was less sensitive to teniposide and CDDP than control cell lines and the number of apoptotic cells after chemotherapy by teniposide and CDDP was reduced. Conclusions:The expression of exogenous p16 gene could inhibit the growth of glioma. On the other hand, the induction of apoptosis by teniposide and CDDP was inhibited in wt p1 positive U251 and its chemosensitivity to teniposide and CDDP was decreased.