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黄芪注射液对烟碱损伤人脐静脉内皮细胞的保护作用
引用本文:涂春香,谢佐福,陈江荣,陈小明,叶小包.黄芪注射液对烟碱损伤人脐静脉内皮细胞的保护作用[J].福建中医学院学报,2011,21(4):19-20.
作者姓名:涂春香  谢佐福  陈江荣  陈小明  叶小包
作者单位:1. 福建中医药大学中西医结合学院,福建福州,350108
2. 江西省上饶卫生学校,江西上饶,334100
基金项目:福建省自然科学基金(2009J01167)
摘    要:观察黄芪注射液对烟碱损伤人脐静脉内皮细胞(HUVEC)的保护作用及分子机制.方法 MTT法检测烟碱、黄芪对HUVEC细胞生长的影响,硝酸还原酶法检测总NO水平、化学比色法检测HUVEC细胞总NOS水平、酶联免疫法检测ET-1的水平. 结果烟碱作用后的HUVEC细胞总NO水平和总NOS水平均降低显著(P<0.01),而...

关 键 词:黄芪  烟碱  人脐静脉细胞

Protective Effects of Astragalus Injection on Human Umbilical Vein Endothelial Cells Injured by Nicotine
TU Chunxiang,XIE Zuofu,CHEN Jiangrong,et al.Protective Effects of Astragalus Injection on Human Umbilical Vein Endothelial Cells Injured by Nicotine[J].Journal of Fujian College of Traditional Chinese Medicine,2011,21(4):19-20.
Authors:TU Chunxiang  XIE Zuofu  CHEN Jiangrong  
Institution:TU Chunxiang1,XIE Zuofu1,CHEN Jiangrong2,et al(1.College of Integrative Medicine,FUTCM,Fuzhou,Fujian 350108,China,2.Shangrao Health school,Shangrao,Jiangxi 334100,China)
Abstract:Objective To observe the protective effect of Astragalus injection on human umbilical vein endothelial cells(HUVEC) injured by nicotine and explore its molecular mechanism.Methods The effects of nicotine,Astragalus on the growth of HUVEC were detected respectively by MTT method,the levels of total NO,total NOS and ET-1 were detected by nitrate reductase assay,chemical colorimetry and enzyme linked immunosorbent assay respectively.Results The levels of total NO and total NOS were decreased significantly,and ...
Keywords:Astragalus  nicotine  human umbilical vein endothelial cells  
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