Cytotoxic T lymphocytes versus streptococcal colonization in periapical granulomas. |
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Authors: | N I Hren M Gubina A Ihan |
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Affiliation: | Clinic for Maxillofacial and Oral Surgery, University Clinical Centre Ljubljiana, Slovenia. |
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Abstract: | Seventeen dental periapical lesions were investigated to study bacterial colonization. Periapical lesions, obtained after apicotomy, were also enzymatically desegregated to quantitatively analyze lymphocyte subpopulations by flow cytometry. Fourteen samples yielded a positive bacterial growth when homogenized and cultured. We isolated enough lymphocytes to make flow cytometric analysis from 12 samples. A significant increase in interleukin-2 receptor and ICAM-1 molecule expression on T cells was found, compared with peripheral blood lymphocytes. Furthermore, a decreased expression of interleukin-2 receptors and HLA DR molecules on CD8+ T cells was found in granulomas predominantly colonized by Streptococcus spp., compared with lesions predominantly colonized by strict anaerobes. |
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