| PAI-1在鼻咽癌细胞系(CNE-2Z)及其克隆株(CNE-2Z-H5,CNE-2Z-H5-9)中表达和意义 |
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| 引用本文： | 潘国庆,付红梅,边莉,金树珍,唐慰萍. PAI-1在鼻咽癌细胞系(CNE-2Z)及其克隆株(CNE-2Z-H5,CNE-2Z-H5-9)中表达和意义[J]. 中国癌症杂志, 2005, 15(3): 271-274 |
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| 作者姓名： | 潘国庆 付红梅 边莉 金树珍 唐慰萍 |
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| 作者单位： | 1. 昆明医学院第一附属医院病理科,云南,昆明,650031
2. 广东医学院病理教研室,广东,湛江,524023 |
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| 摘 要： | 目的:探讨尿激酶激活剂抑制剂-1(Plasminogenactivatorinhibitor-1,PAI-1)在不同淋巴道转移能力的鼻咽癌细胞系(CNE-2Z)及其克隆株(CNE-2Z-H5,CNE-2Z-H5-9)中的表达和意义。方法:应用逆转录聚合酶反应(RT-PCR)方法检测PAI-1在基因转录水平的表达;使用异质粘附、侵袭抑制实验观察PAI-1在鼻咽癌细胞侵袭和转移过程中的作用。结果:PAI-1在CNE-2Z,CNE-2Z-H5中均有转录,但无显著性差异;PAI-1在CNE-2Z-H5-9中无转录。抗PAI-1抗体对CNE-2Z-H5-9异质粘附、侵袭无影响。结论:PAI-1可能抑制鼻咽癌的侵袭和转移。
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| 关 键 词： | 尿激酶激活剂抑制剂-1 鼻咽癌 侵袭、转移和异质粘附 |
| 文章编号： | 1007-3639(2005)03-0271-04 |
| 修稿时间： | 2004-09-28 |
Expression and significance of PAI-1 in nasopharyngeal carcinoma (NPC) cell line(CNE-2Z) and its sub-line (CNE-2Z-H5,CNE-2Z-H5-9) |
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| PAN Guo-qing,FU Hong-mei,BIAN Li,et al. Expression and significance of PAI-1 in nasopharyngeal carcinoma (NPC) cell line(CNE-2Z) and its sub-line (CNE-2Z-H5,CNE-2Z-H5-9)[J]. China Oncology, 2005, 15(3): 271-274 |
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| Authors: | PAN Guo-qing FU Hong-mei BIAN Li et al |
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| Abstract: | Purpose:To investigate the expression and significance of PAI-1 in nasopharyngeal carcinoma (NPC) cell line (CNE-2Z) and its sub-lines (CNE-2Z-H5, CNE-2Z-H5-9) with different capabilities of lymph node metastasis. Methods:RT-PCR was used to detect expression of PAI-1 at levels of mRNA; The experiments of inhibiting heterogeneous adhesion and invasion were used to observe the role of PAI-1 in PNC's metastasis and invasion. Results:expression of PAI-1 was detected in CNE-2Z, CNE-2Z-H5, but there was not significant difference between them (P>0.05) ; whereas it was seen in CNE-2Z-H5-9. Anti-PAI-1 antibody did not inhibit the heterogeneous adhesion and invsion of CNE-2Z-H5-9. Conclusions:PAI-1 maybe inhibit the metastasis and invasion of NPC. |
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| Keywords: | PAI-1 NPC cell line Invasion metastasis and heterogeneous adhesion |
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