硝普钠对培养的猪主动脉平滑肌细胞增殖的影响

内皮细胞损伤和血管平滑肌细胞增殖在动脉粥样硬化发生发展过程中起关键作用，本工作用离体冠状动脉实验证明硝普钠呈剂量依赖性松弛冠状动脉，且与内皮去除与否无关。用噻唑蓝比色法观察到硝普钠也能剂量依赖性抑制主动脉平滑肌细胞增殖。结果表明，ＮＯ对平滑肌细胞增殖具有调节作用。提示在临床上对于动脉粥样硬化及冠心病病人，长期使用硝普钠等与ＮＯ合成及释放有关的硝基化合物不仅可以缓解冠状动脉痉挛，而且还可抑制平滑肌细胞增殖，可能有助于延缓动脉粥样硬化的进一步发展。

Effects of Sodium Nitroprosside on proliferation of Cultured Porcine Aortic Smooth Muscle Cells

Vascular endothelial Cell damage damage and excessive coth muscle cell(SMC)proliferation are considered to paly a key role in the development of a atherosclerotic disease.In present study,it was ob.served that nitric oxide-generating vasodilator-sodium nitroprosside(SNP)induced endothelium-independent relaxation on porcine coronary arterial rings and inhibited mitogenesis and proliferation ofcultured procine vascular smooth muscle cells in dose-dependent manner with MTT colorimetric assay, The results suggest that endogenous nitric oxide(NO)may play an important role as medulatorof smooth muscle cell growth,and the long-term administration of SNP and other nitrovasodilators which release NO in the they favors inhibiting the development of atherosclerosis to patients with atherosclerotic disease.