母血中胎儿有核红细胞数量与胎儿异常的关系

目的 :探讨母血中胎儿有核红细胞数量与胎儿异常的关系 ,为将它用于无创性产前诊断提供实验依据。方法 :对孕 6～ 40周的 86例妇女 (包括胎儿正常组 68例、胎儿异常组 1 8例 )的外周血进行单密度梯度离心、瑞氏染色和细胞计数 ,分析母血中胎儿有核红细胞数量与孕周、胎儿异常及胎儿性别的关系。结果 :胎儿正常组 ,母血中胎儿有核红细胞平均数量为 1 4.2 3±1 2 .0 1 /ml,与妊娠周数呈直线相关 (R >R0 .0 5)。胎儿异常组中 ,母血中胎儿有核红细胞平均数量较正常胎儿组显著增加 ,约为 3 8.73± 2 4.97/ml,且与妊娠周数无明显直线相关性。母体外周血中胎儿有核红细胞数量与胎儿性别无统计学相关性。结论 :胎儿发生异常时母血中胎儿有核红细胞数量较正常妊娠时增加。母血中胎儿有核红细胞数量在胎儿正常时随孕周而增加 ,且不受胎儿性别的影响。母血中胎儿有核红细胞计数可以辅助用于产前筛查胎儿异常的发生。

The relationship between the quantity of fetal nuclear red blood cells and abnormal fetus

Objective:To explore the quantity of fetal nuclear red blood cells (FNRBC) in maternal peripheral blood and its relationship with abnormal fetus,providing experimental support to the utility of FNRBC in the non traumatic prenatal diagnosis.Methods:The quantity of FNRBC was counted after distinguishing it by single density centrifugation and Wright's staining in 86 pregnant women in 8～40 weeks.The relationship among pregnant weeks,abnormal fetus and fetal sex was analysed.Results:The average quantity of FNRBC in normal fetus group was 14.23±12.01/ml and had a significant relativity with pregnant week.In abnormal fetus group, the quantity of FNRBC was significantly higher than that in normal fetus group and had no direct relativity with pregnant week.The quantity of FNRBC had no statistic relativity with fetus sex.Conclusion:When the fetus is abnormal,the quantity of FNRBC in maternal peripheral blood becomes absolutely higher than that in normal fetus.The quantity of FNRBC in maternal peripheral blood increases with the pregnant week and is not affected by fetus sex.The account of the FNRBC in maternal peripheral blood can help screen out abnormal fetus in prenatal diagnosis.