精索内动静脉高位结扎iNOS的表达与睾丸生精细胞增殖和凋亡的关系

目的：探讨精索内动静脉高位结扎术后，诱导型一氧化氮合酶（inducible nitric oxide synthase．iNOS）的表达与生精细胞增殖和凋亡的关系。方法：免疫组化结合计算机图像分析系统检测iNOS阳性细胞光密度值；免疫组化方法检测并计算PCNA增殖指数（PCNA-Labeling index，PI）；原位末端标记法（terminal deoxynucleotidyl transferase mediated dUTP nick-endlabeling method，TUNEL）检测生精细胞凋亡指数（apoptosis index，AI）。分析iNOS的表达与增殖、凋亡的相关性。结果：iNOS在术后3-15d表达增强，第7d呈强阳性表达。结扎组生精细胞PCNA增殖指数（PI）明显低于对照组（P〈0．01），凋亡细胞指数（AI）明显高于对照组（P〈0．01）。结论：精索内动静脉高位结扎，iNOS的表达与生精细胞PCNA增殖指数呈负相关，与生精细胞凋亡指数呈正相关。iNOS基因表达增强是生精细胞增殖减少和凋亡增加的重要原因。

The relationships of iNOS expression with spermatogenic cell proliferation and apoptosis following high ligation of internal spermatic cord vessels

Objective: To investigate the relationships of iNOS expression with spermatogenic cell proliferation and apoptosis following high ligation of internal spermatic cord vessels. Methods: iNOS was detected by immunohistochemistry combined with computer-assisted image analysis system. PCNA-labeling index(PI) was determined and calculated by immunohistochemistry, while spermatogenic cell apoptosis index(AI) was determined by TUNEL(terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling). The relationships of iNOS expression with spermatogenic cell proliferation and apoptosis were analyzed. Results: iNOS expression increased 3-15 days following the high ligation, the high-positive expression of iNOS was detected on the 7th day after operation. PCNA-index(PI) in the high ligation groups were much lower than the control groups(P<0.01), while apoptosis index(AI) were higher than the control groups (P<0.01). Conclusions: After high ligation of internal spermatic cord vessels, the iNOS expression has negative correlation with PI of spermatogenic cell, and has positive correlation with AI. The over-expression of iNOS gene is one of the major reasons for decreasing of spermatogenic cell proliferation and increasing of apoptosis.