甘利欣对大鼠哮喘模型及哮喘患者血清IgE水平的影响

目的：探讨甘利欣对哮喘模型及患者血清中IgE水平的影响。方法：将大鼠随机分为正常对照组、哮喘模型组、甘利欣(GL)用药组及地塞米松(DS)阳性对照组。哮喘模型制备用卵白蛋白(OVA)致敏大鼠并雾化吸入刺激。模型建立成功指标采用描记呼吸曲线、肺组织病理切片及血清IgE水平的变化。除动物模型外并观察了哮喘息者血清IgE的水平。结果：哮喘模型组呼吸曲线的呼吸幅度变深，节律变快，肺组织病理切片HE染色显示有较多炎症细胞浸润，杯状细胞增生明显，平滑肌增厚。血清IgE较正常组明显增高，两组比较差异显著(P＜0．005)。甘利欣用药组呼吸曲线的呼吸幅值变小，肺组织病理切片HE染色支气管壁炎症细胞浸润减轻，无大量杯状细胞增生，血清IgE检测较模型组减低，两组比较差异显著(P＜0．01)，但与地塞米松(DS)阳性对照治疗组比较无差异。哮喘患者血清IgE治疗前明显高于正常水平(P＜0．01)，甘利欣用药后明显降低。结论：甘利欣能降低大鼠哮喘模型及哮喘息者的血清IgE水平。

The effect of Glycrrhizinatis on serum IgE level of asthmatic rat model and patients with asthma

Objective:To explore the effect of glycynhizinatis on serum IgE level of rat model and patients with asthma.Methods:Trie rats were divided into four groups randomly: normal control, asthmatic model, model treated with GL, model treated with DS. The rats were sensitized and challenged with ovalbumin to establish an asthmatic model. The established models were measured by breathing curves, pathological changes in pulmonary tissue slices and the level of serum IgE. Furthermore, serum IgE level of patients with asthma was also detected. Results: High amplitude of breathing curve and fast rhythm were showed in group of asthmatic model, there were more inflammatary cells infiltration in the tiny bronchial wall, hyperplasia of cup-shaped cells and thicker smooth muscle in the pulmonary tissue slices. Compared with normal control, the level of serum IgE in model group increased obviously. The rats treated with glycyrrhizinatis showed low amplitude of breathing curve, decreasing inflammatary cells infiltration in pulmonary tissue slices of tiny bronchial wall, no hyperplasia of cup-shaped cells, and decreased serum IgE level. There were no significant difference between the groups of GL and DS. At the same time, the serum IgE level in patients with asthma was observed. The results showed that the level of serum IgE was higher in patients with asthma than that in normal control (P < 0.01). Conclusion: Glycyrrhizinatis can decrease the level of serum IgE of asthmatic rat model and patients with asthma.