头孢他啶在甲硝唑葡萄糖注射液中的稳定性

目的：本文模拟临床头孢他啶使用习惯，将头孢他啶加入甲硝唑葡萄糖注射液中静滴，研究其稳定性。方法：根据两种药物的pH范围，将甲硝唑葡萄糖注射液调整pH为4．51和6．06，后模拟临床用药剂量，取头孢他啶2g分别加入250mL上述两种液体中，混匀得Ⅰ、Ⅱ号液，取100灿加过滤蒸馏水稀释至10mL，进样20μL。用高效液相色谱法，在检测波长254nm，流动相5％甲醇，流速0．70mL-min^-1，灵敏度0．05AUFS，柱温20℃；25℃和35℃的条件下检测两种药物的稳定性。结果：两种药物在不同条件下、不同时间段药物浓度含量变化在±10％以内。结论：头孢他啶与甲硝唑葡萄糖注射液伍用，25℃、4h或35℃、2h，其含量变化不明显，pH略有下降，不影响用药安全、有效。

Stability of ceftazidime in metronidazole and glucose injection

Objective： To study the stability of ceftazidime in metronidazole and glucose injection by mimicing the clinical usage of these drugs. Methods： According to the pH ranges of these two drugs, metronidazole and glucose injection was adjusted to pH 4.51 and 6.06. Then according to clinical usage dose, 2 g ceftazidime was added into 250 mL solutions with two different pH values. 100 μL solutions mentioned above was diluted to 10 mL with distilled water. Then 20 μL was taken as sample. The stability of the drugs was detected by high performance liquid chromatography （HPLC）. The detection wavelength was 254 nm, the mobile phase was 5% methanol, the flow rate was 0.70 mL·min^-1 and the sensitivity was 0.05 AUFS. The column temperature was 20℃ and the experiments were carried out at 25 ℃ and 35 ℃. Results： The contents of the two drugs changed within ±10% under different conditions. Conclusion： The two drugs contents did not change and the pH value decreased slightly during 4 h in 25 ℃ or 2 h in 35℃. It did not affect the effectiveness and safety of ceftazidime in metronidazole and glucose injection.