长链非编码RNA XLOC_002319在贲门腺癌中的表达及其甲基化状态

目的]检测贲门腺癌(GCA)中长链非编码RNA XLOC_002319(lncRNA XLOC_002319)的表达及其甲基化状态,探讨XLOC _002319在贲门腺癌发生发展中的作用.方法]分别应用实时荧光定量聚合酶链反应(qRT-PCR)以及甲基化特异性PCR(MSP)检测贲门腺癌组织、癌旁不典型增生组织及癌旁正常组织中XLOC_ 002319的表达和甲基化状态.结果]XLOC_002319在贲门腺癌组织和癌旁不典型增生组织中的表达显著低于癌旁正常组织(P＜0.01),并且在贲门腺癌组织中XLOC_ 002319的表达与组织学分化程度、淋巴结转移和TNM分期密切相关(P＜0.05).贲门腺癌组织中XLOC _002319的启动子区甲基化率(61.54％)和癌旁不典型增生组织甲基化率(54.84％)显著高于癌旁正常组织(17.95％)(P＜0.01),并且贲门腺癌组织中XLOC_002319的启动子区甲基化率与组织学分化程度、淋巴结转移和TNM分期密切相关(P＜0.05).发生XLOC_002319甲基化的贲门腺癌组织中XLOC_002319的表达显著低于未发生甲基化的贲门腺癌组织(0.217±0.074 vs 0.253±0.060,P＜0.05).结论]XLOC_002319在贲门腺癌中的异常低表达可能与贲门腺癌的发生密切相关,且其启动子区甲基化可能是导致其表达沉默的机制之一.

Expression and Methylation Status of LncRNA XLOC_002319 in Gastric Cardia Adenocarcinoma

Purpose] To investigate the expression and methylation status of lncRNA XLOC_002319 in GCA(gastric cardia adenocarcinoma),and to elucidate its role in GCA.Methods] qRT-PCR and MSP methods were used respectively to detect the expression and methylation status of XLOC_002319 in GCA tissues,atypical hyperplasia tissues and corresponding non-cancerous tissues.Results] The expression of XLOC_002319 in GCA tumor tissues and atypical hyperplasia tissues was significantly reduced compared to corresponding non-cancerous tissues (P＜0.01),and the expression of XLOC_002319 in GCA tumor tissues was associated with lymph node,pathological differentiation and TNM stage (P＜0.05).The methylation frequency of XLOC_002319 promoter in GCA tissues(61.54％) and atypical hyperplasia tissues(54.84％)was significantly higher than that in corresponding normal tissues(17.95％)(P＜0.01),and the methylation frequency of XLOC_002319 promoter in GCA tissues was also associated with lymph node,pathological differentiation and TNM stage (P＜0.05).The expressions of XLOC_002319 in GCA tumor tissues with methylation of the gene was significantly lower than that in tumor tissues with unmethylation of the gene(0.217±0.074 vs 0.253±0.060,P＜0.05).Conclusions] Aberrant low expression of lncRNA XLOC_002319 is closely related to the development and occurrence of GCA,and promoter methylation may be one of the mechanisms for inactivation of XLOC_002319 in GCA.