Evaluation of endocrine disrupting activity of plasticizers in polyvinyl chloride tubes by estrogen receptor alpha binding assay |
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Authors: | Atsushi Ohashi PhD Hirohisa Kotera BS Hideo Hori BS Makoto Hibiya PhD Koji Watanabe MD PhD Kazutaka Murakami MD PhD Midori Hasegawa MD PhD Makoto Tomita MD PhD Yoshinobu Hiki MD PhD Satoshi Sugiyama MD PhD |
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Institution: | (1) Department of Clinical Biochemistry, Fujita Health University College, 1-98 Kutsukake-cho, Toyoake, Aichi 470-1192, Japan;(2) Department of Clinical Laboratory, Kyoto City Hospital, Kyoto, Japan;(3) Department of Nephrology, Graduate School of Medicine, Fujita Health University, Toyoake, Japan |
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Abstract: | Polyvinyl chloride (PVC) tubing is an indispensable medical material for extracorporeal circulation therapy. However, di(2-ethylhexyl)phthalate
(DEHP), a suspected endocrine disruptor, can be eluted from PVC, suggesting that an alternative material that does not contain
DEHP is needed for clinical applications. First, we evaluated the endocrine disrupting risks of the plasticizers contained
in PVC tubes by investigating their binding affinities for the human estrogen receptor alpha (ERα). Our results revealed that,
while DEHP has some binding affinity for ERα, neither epoxidized soybean oil nor tris(2-ethylhexyl)trimellitate (an alternative
to DEHP) has any affinity for ERα. Second, we evaluated the endocrine disrupting risks of a tube made of newly developed plasticizer-free
(PF) materials. We confirmed the presence of DEHP and detected several unidentified substances in plasma stored within the
PVC tube. This plasma's competitive binding affinity for ERα was significantly higher than that of control plasma (P < 0.01). In contrast, the profile of plasma stored in the PF tube was similar to that of the control, both in terms of high-performance
liquid chromatography chromatograms and competitive binding capacity for ERα, suggesting that the PF tube is biocompatible
and is useful for reducing the elution of substances capable of binding to ERα.
Presented in part at the 42nd Congress of the Japanese Society for Artificial Organs, October 5–7, 2004, Tokyo, Japan |
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Keywords: | Endocrine disruptor Extracorporeal circulation tube Estrogen receptor alpha Di(2-ethylhexyl)phthalate Tris(2-ethylhexyl)trimellitate Plasticizer-free materials |
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