狼疮肾炎患者血清自身抗体检测及意义

目的探讨狼疮肾炎（LN）患者血清自身抗体检测的临床意义。方法检测60例系统性红斑狼疮（SLE）患者血清抗Clq抗体、ANA抗体、抗dsDNA、核小体、抗一Sm抗体，并依据有无狼疮肾炎分为狼疮肾炎组（A组）38例和无狼疮肾炎的SLE对照组（B组）22例。结果A组抗C1q、dsDNA、核小体、抗-Sm和ANA抗体阳性率显著高于B组（均P〈0．01），血清中抗C1q抗体水平分别与SLEDAI评分、抗ANA水平呈显著正相关（均P〈0．01）；与补体C3、C4含量呈显著负相关（P〈0．01）。结论抗C1q抗体、dsDNA、核小体和ANA是反映SLE患者并发肾脏损害的重要指标，在LN诊断和判定活动性方面有重要作用。

Clinical significance of autoimmune antibody in patients with lupus nephritis

Objective To investigate the clinical significance autoimmune antibody in patients with lupus nephritis(LN).Methods ELISA assay was performed to examine anti-C1q antibody and anti-nucleus antibody;immunoblot assay was performed to examine anti-dsDNA antibody,anti-nucleosome antibody and anti-Sm antibody in sera of 38 patients with LN and 22 patients of system lupus erythemalosus without renal damage.Standard methods were uaed for laboratory testing.Results The positive rate of anti-C1q antibody,anti-dsDNA antibody,anti-nucleosome antibody,anti-Sm antibody and anti-nucleus antibody were significantly higher in LN than in controls(P＜0.01).AntiC1q antibody and anti-nucleus antibody were significantty higher in LN than in controls(P＜0.01,P＜0.05).Serum levels of anti-C1q antibody was positively correlated with SLEDAI score and the level of anti-nucleus antibody,but was negatively oorrelated with C3、 C4.Conclusion Anti-C1q antibody,anti-dsDNA antibody,anti-nucleosome antibody and anti-nucleus antibody are suggested to play a role in the pathogenesis of LN and can be used as diagnostic tools and disease activity markers.