| 从Cx26探究六味地黄丸增效肝癌自杀基因治疗的机制 |
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| 引用本文： | 易华,杜标炎,谭宇蕙,孙军,吴映雅,李杰芬,郭晶.从Cx26探究六味地黄丸增效肝癌自杀基因治疗的机制[J].中华中医药杂志,2009(9). |
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| 作者姓名： | 易华 杜标炎 谭宇蕙 孙军 吴映雅 李杰芬 郭晶 |
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| 作者单位： | 广州中医药大学基础医学院;暨南大学水生生物研究所;广州中医药大学; |
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| 基金项目： | 国家自然科学基金资助项目(No.30572433) |
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| 摘 要： | 目的:探讨六味地黄丸对大鼠肝癌CBRH7919细胞Cx26表达的影响,初步阐明其对自杀基因治疗的增效作用是否与缝隙连接机制相关。方法:应用血清药理学研究方法制备六味地黄丸含药血清。采用MTT法检测六味地黄丸含药血清联合单纯疱疹病毒-胸苷激酶/丙氧鸟苷(HSV-tk/GCV)自杀基因系统治疗的杀伤效应:应用蛋白印迹(Western blot)技术、间接免疫荧光法及荧光定量逆转录-聚合酶链反应(RT-PCR)技术检测其对CBRH7919细胞株Cx26蛋白及mRNA表达的影响。结果:①六味地黄丸含药血清联合自杀基因治疗组肝癌细胞存活率明显低于单纯自杀基因组(10%tk+/GCV加对照血清)(P<0.01),且联合作用均具有协同性(Q>1.15);②Western blot检测显示六味地黄丸含药血清能提高Cx26蛋白的表达,并有明显的量效关系;③间接免疫荧光法(FITC)法共聚焦显微镜观测显示近似的结果:蛋白定位于细胞膜和胞浆,尤其在细胞膜的表达明显增多,含药血清组的Cx26蛋白荧光强度比对照组明显增加(P<0.05)。结论:六味地黄丸增强自杀基因旁杀伤效应的机制与其促进肝癌细胞Cx26 mRNA及蛋白表达,增加膜上...
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| 关 键 词： | 肝癌 六味地黄 基因治疗 血清药理学 缝隙连接 |
Investigate the mechanism of Liuwei Dihuang Bolus enhancing hepatocarcinoma suicide gene therapy from Cx26 |
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| YI Hua,DU Biao-yan,TAN Yu-hui,SUN Jun,WU Ying-ya,LI Jie-fen,GUO Jing.Investigate the mechanism of Liuwei Dihuang Bolus enhancing hepatocarcinoma suicide gene therapy from Cx26[J].China Journal of Traditional Chinese Medicine and Pharmacy,2009(9). |
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| Authors: | YI Hua DU Biao-yan TAN Yu-hui SUN Jun WU Ying-ya LI Jie-fen GUO Jing |
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| Institution: | YI Hua1,DU Biao-yan1,TAN Yu-hui1,SUN Jun2,WU Ying-ya1,LI Jie-fen1,GUO Jing3(1Pathology Department.of Preclinical College of Guangzhou University of Chinese Medicine,Guangzhou 510006,China,2Jinan University Aquatic Research Institute,Guangzhou 510630,3Guangzhou University of Chinese Medicine,Guangzhou 510405,China) |
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| Abstract: | Objective:To investigate the regulation effect of medicated serum of Liuwei Dihuang Bolus on the expression of Connexin26 in rat hepatocarcinoma CBRH7919 cell,and clarity whether the mechanism of Liuwei Dihuang Bolus promoting the suicide gene therapy was related with the gap junction.Methods:Blood-serum pharmacology was used to prepare the medicated serum of Liuwei Dihuang Bolus.The lethal e ect of the medicated serum of Liuwei Dihuang Bolus associated with the HSV-tk/GCV suicide gene therap system was det... |
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| Keywords: | Hepatocarcinoma Liuwei Dihuang Bolus Gene therapy Serum pharmacology Gap junction |
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