子宫颈癌组织中人乳头状瘤病毒16 E6 mRNA表达与survivin蛋白表达的相关性

目的探讨宫颈癌组织中人乳头状瘤病毒(HPV)16E6mRNA表达与survivin蛋白表达的相关性。方法采用半定量PCR技术和免疫组化链霉菌抗生物素蛋白-过氧化物酶连接法,检测慢性宫颈炎、宫颈上皮内瘤变(CIN)及宫颈癌共148例患者宫颈组织中HPV16E6mRNA及survivin蛋白的表达。结果148例患者中,HPV16阳性共37例,其中慢性宫颈炎5例、CINⅠ6例、CINⅡ～Ⅲ11例及宫颈癌15例。慢性宫颈炎、CINⅠ、CINⅡ～Ⅲ及宫颈癌组织中,HPV16E6mRNA的表达水平分别为0.3±0.4、0.6±0.4、1.8±0.6及2.4±0.6;survivin蛋白阳性表达率分别为7%、31%、63%及84%。CINⅡ～Ⅲ及宫颈癌组织中,HPV16E6mRNA的表达水平及survivin蛋白阳性表达率均显著高于慢性宫颈炎及CINⅠ组织,两者比较,差异均有统计学意义(P<0.01)。宫颈癌组织中,HPV16E6mRNA的表达水平与survivin蛋白阳性表达率呈显著正相关关系(γs=0.62,P<0.05)。结论HPV16E6mRNA的表达水平与宫颈病变的进展有关,survivin蛋白阳性表达率升高可能与宫颈癌的发生、发展密切相关。

Quantities of human papillomavirus 16 E6 mRNA and its relationship with expression of survivin in cervical cancer

OBJECTIVE: To investigate the quantities of human papillomavirus (HPV) 16 E6 gene and to study its relationship with the expression of survivin in cervical carcinoma. METHODS: In 148 patients including cervical intraepithelial neoplasia (CIN), cervical carcinoma and chronic cervicitis, the quantities of HPV 16 E6 gene were detected by semiquantitative polymerase chain reaction (PCR); and the expressions of survivin were measured by immunohistochemical streptavidin-biotin peroxidase (SP) assay. RESULTS: The quantities of HPV 16 E6 gene were 0.3 +/- 0.4, 0.6 +/- 0.4, 1.8 +/- 0.6 and 2.4 +/- 0.6, and expression rates of survivin were 7%, 31%, 63% and 84% respectively in chronic cervicitis, CIN I, CIN II-III and cervical cancer tissues. The quantities of HPV 16 E6 gene and the expressions of survivin tended to increase along with the tumor progression (P < 0.05). The quantities of HPV16 E6 gene and the expressions of survivin of CIN I, II-III or cervical cancer were significantly higher than that of chronic cervicitis or CIN I (P < 0.01). Positive rank correlation between the quantities of HPV16 E6 gene and the expression of survivin was observed in cervical carcinoma (gamma(s) = 0.62, P < 0.05). CONCLUSIONS: The quantities of HPV16 E6 gene appear to be associated with an increased risk of developing cervical cancer. Survivin overexpression might play an important role in the process of cervical carcinoma.