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Intramolecular recombination of a mitochondrial minicircular plasmid-like DNA of date-palm mediated by a set of short direct-repeat sequences
Authors:Abdel Ali Benslimane  Caroline Hartmann  Bouchra Ouenzar  André Rode
Affiliation:(1) Département de Biologie, Faculté des Sciences Semlalia, Université Cadi Ayyad, BP S15, 40001 Marrakech, Maroc;(2) Institut de Biotechnologie des Plantes, Université de Paris Sud, URA CNRS 1128, Batiment 630, F-91405 Orsay, France
Abstract:A molecular clone containing the complete sequence of a mitochondrial circular plasmid-like DNA (the R plasmid) isolated from the date-palm variety V3DP was used as a probe in Southern analyses of mitochondrial DNA prepared from other varieties. Another circular structure (the S plasmid) was detected in some of these varieties, and sequenced from variety V2DP. It appears that the R plasmid could have arisen from the S plasmid by an intermolecular recombination event at a set of 26-bp imperfect short direct repeats.
Keywords:Mitochondrial DNA  Plasmid-like DNA  Date-palm  Recombination
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