气相色谱-质谱和化学计量学解析法分析药对麻黄-桂枝挥发油成分

利用气相色谱-质谱对药对麻黄-桂枝及单味药麻黄和桂枝的挥发油成分进行检测，通过化学计量学解析法对产生的二维色谱-质谱数据进行解析，得到各组分的纯色谱和质谱，根据其保留时间和质谱，在质谱库中进行相似检索，实现对组分的定性，再用总体积积分法进行定量。麻黄-桂枝、麻黄和桂枝挥发油分别定性了97，72和68个色谱峰，占总含量的89.76%，90.08%和91.62%。药对挥发油成分的数目大致为麻黄和桂枝挥发油成分的加和，但相对含量有变化。

Analysis of essential oil in Herba Ephedrae-Ramulus Cinnamomi by GC-MS and chemometric resolution method

Gas chromatography/mass spectrometry,chemometric resolution method(CRM)and overall volume integration method were used to analyze the essential components of herbal pair Herba Ephedrae-Ramulus Cinnamomi(HP HE-RC)and compare it with that of single herbs HE and RC.72,68,and 97 essential components in essential oil of HE,RC,and HP HE-RC were determined,accounting for 90.08%,91.62%,and 89.76% of total contents of essential oil of HE,RC,and HP HE-RC,respectively.The numbers of essential components of HP HE-RC are almost the summation of that of two single herbs,but some relative contents of them are changed.Some new components,such as 1,6-dimethylhepta-1,3,5-triene,tetracyclo4.2.1.1(2,5).0(9,10)]deca-3,7-diene,globulol,(E,E)-6,10,14-trimethyl-5,9,13-pentadecatrien-2-one,etc.have been found in HP HE-RC because of chemical reactions and physical changes during the course of decocation.