微渗析结合RP-HPLC研究盐酸平阳霉素在家兔血中的药代动力学

采用微渗析技术研究盐酸平阳霉素在体药代动力学，用Supelco RP-amide C₁₆柱进行样品的HPLC分析，盐酸平阳霉素的血药浓度用3P87程序处理。结果表明，家兔静注盐酸平阳霉素后，渗析液中的药物浓度在1.04～66.56 μg·mL^-1时，浓度与峰面积有良好的线性关系(R²=0.999 4)。微渗析探针的在体回收率为(42.8±3.4)%(n =6)。所测血药浓度经3P87程序拟合后，药-时曲线符合二室模型，T_1/2α及T_1/2β分别为14.9及60.3 min。因此，该方法可用于盐酸平阳霉素药代动力学研究。

Pharmacokinetics of pingyangmycin hydrochloride in rabbits determined by microdialysis coupled with RP-HPLC

Microdialysis coupled with RP-HPLC was used to study the blood pharmacokinetics of pingyangmycin hydrochloride in rabbits. Supelco RP-amide C16 column was adopted for the analysis of pingyangmycin hydrochloride. The data was analyzed with 3P87 program. The calibration curve was linear in the concentration range from 1.04 to 66.56 microg x mL(-1) (r2 = 0.999 4). The in vivo recovery of microdialysis probe was (42.8 +/- 3.4)% (n = 4). The concentration-time curve of pingyangmycin hydrochloride was fitted to two-compartment model. T1/2 alpha and T1/2 beta were 14.9 and 60.3 min, respectively. The method is proved to be accurate, simple and suitable for the pharmacokinetics study of pingyangmycin hydrochloride in rabbits.