HPLC-UV-ELSD法同时测定青蒿中青蒿素、青蒿乙素和青蒿酸的含量

用HPLC-UV-ELSD法同时测定青蒿药材中青蒿素、青蒿乙素和青蒿酸的含量。采用Nucleodur C₁₈色谱柱(250 mm×4.6 mm，5 μm ID)；以乙腈-0.1%乙酸水(50∶50)为流动相；紫外检测波长209 nm,蒸发光散射检测器漂移管温度50 ℃。结果显示，青蒿素、青蒿乙素和青蒿酸能够达到很好分离。它们的线性范围分别为0.52～2.6 μg， r=0.999 4(n=5)； 0.022～4.4 μg， r=0.999 9(n=5)； 0.203～8.12 μg，r=0.999 8(n=5)。平均回收率分别为99.45%(RSD=2.3%， n=6)；102.37%(RSD=1.7%， n=6)；101.10%(RSD=0.79%， n=6)。本法简单、准确、快速，可同时测定青蒿药材中青蒿素、青蒿乙素和青蒿酸的含量。

Determination of artemisinin, arteannuin B and artemisinic acid in Herba Artemisiae Annuae by HPLC-UV-ELSD

To establish an HPLC-UV-ELSD method for the determination of the content of artemisinin, arteannuin B and artemisinic acid in Herba Artemisiae Annuae. The analytical column was Nucleodur RP-C18 (250 mm x 4.6 mm, 5 microm ID). The mobile phase was acetonirile-0.1% acetic acid (50: 50) and the flow rate was 1.0 mL x min(-1) with a UV detector for artemisinin, the detection wavelength at 209 nm, and the evaporative light-scattering detector (ELSD) for arteannuin B and artemisinic acid, the drift tube temperature: 50 degrees C, the nitrogen flow rate 30 psi and the gain was 50. The resolution of artemisinin, arteannuin B and artemisinic acid was good. The linear calibration curves were obtained over the range of 0.52 - 2.6 microg for artemisinin (r = 0.999 4, n = 5), 0.022 - 4.4 microg for artemisinin B (r = 0.999 9, n = 5) and 0.203 - 8.12 microg for artemisinic acid (r = 0.999 8, n = 5), separately. The mean recoveries of the three compounds were 99.45%, 102.37% and 101.10% with RSD of 2.3%, 1.7% and 0.79%, respectively. This method is simple, rapid, accurate and suitable for the determination of the content of the three compounds in the herbs.