蛇床子药材的高效液相色谱指纹图谱

采用液相色谱-质谱联用技术对蛇床子药材的活性成分进行定性分析，建立评价其质量的指纹图谱分析方法。采用Shimadzu C₁₈色谱柱，以乙腈-0.1%醋酸水溶液为流动相梯度洗脱，流速为1.0 mL·min^-1，检测波长为245 nm和322 nm，测定了35批不同来源的蛇床子药材的HPLC-UV指纹图谱以及其中4批代表性药材的HPLC-DAD-MS图谱。根据指纹图谱相似度分析结果，将蛇床子药材分为4类；利用HPLC-DAD-MS技术分析了4类药材化学组成上的异同，分别在4类药材中指认了8个，7个，4个和2个香豆素类成分。提示本方法可用于蛇床子药材的指纹图谱测定，并可为其质量评价提供可靠依据。

Chromatographic fingerprint of Cnidium monnieri

To establish the method of HPLC-fingerprint analysis for the quality control of Cnidium monnieri L. Cuss., and identify its active constituents by HPLC-MS, 35 batches of samples were analyzed on a Shimadzu C18 column with a gradient of acetonitrile and 0.1% aqueous aceticacid at a flow rate of 1.0 mL x min(-1) and detected at 245 nm and 322 nm. Furthermore, the typical samples were detected by HPLC-DAD-MS under negative ion mode. 35 batches of Cnidium monnieri L. Cuss. samples were classified into four types based on the results of similarity analysis. According to the comparison of the t(R), MS data and UV maximum absorbance (lambda(max)) values with the standards, 8, 7, 4 and 2 coumarins components were identified in four types of Cnidium monnieri L. Cuss. extracts, separately. The method is repeatable and reliable, and it is capable of effectively controlling the quality of Cnidium monnieri L.