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| 广藿香ITS基因型与地理分布的相关性分析 | |
| 引用本文： | 张英,陈瑶,张金超,杨梦甦,曹晖,肖培根.广藿香ITS基因型与地理分布的相关性分析[J].药学学报,2007,42(1):93-97. |
| 作者姓名： | 张英 陈瑶 张金超 杨梦甦 曹晖 肖培根 |
| 作者单位： | 1. 北京中医药大学,中药学院,北京,100029;香港城市大学,深圳研究院,广东,深圳,518057 2. 香港城市大学,深圳研究院,广东,深圳,518057 3. 香港城市大学,生物及化学系,香港;河北大学,化学与环境科学学院,河北,保定,071002 4. 香港城市大学,生物及化学系,香港 5. 国家中药现代化工程技术研究中心,广东,珠海,519020 6. 中国医学科学院,药用植物研究所,北京,100094 |
| 基金项目： | 国家科技攻关项目;香港城市大学应用研究基金 |
| 摘 要： | 探讨广藿香Pogostemon cablin (Blanco) Benth.的地理分布与ITS基因型的关系，为广藿香的道地性分析和种质评价提供分子依据。采用PCR直接测序技术对不同产地的广藿香ITS1和ITS2基因进行测序分析。结果显示，不同产地广藿香ITS1基因和ITS2基因核苷酸序列长度分别为424 bp和380 bp，其中前者存在19个变异位点，后者存在5个变异位点，说明广藿香的地理分布和其ITS1，ITS2基因型具有良好的相关性，因而ITS1和ITS2测序分析可为广藿香的地理分布与基因型的相关性及其种质评价提供有用的分子信息。 |
| 关 键 词： | 广藿香 DNA测序 种质评价 |
| 文章编号： | 0513-4870（2007）01-0093-05 |
| 收稿时间： | 2006-04-14 |
| 修稿时间： | 2006-04-14 |
Correlation between ITS genotype and geographical distribution of Pogostemon cablin |
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| ZHANG Ying,CHEN Yao,ZHANG Jin-chao,YANG Meng-su,CAO Hui,XIAO Pei-gen.Correlation between ITS genotype and geographical distribution of Pogostemon cablin[J].Acta Pharmaceutica Sinica,2007,42(1):93-97. | |
| Authors: | ZHANG Ying CHEN Yao ZHANG Jin-chao YANG Meng-su CAO Hui XIAO Pei-gen |
| Institution: | 1. School of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beifing 100029, China; 2. Shenzhen Institute of City University of Hong Kong, Shenzhen 518057, China ; 3. Department of Biology and Chemistry, City University of Hong Kong, Hong Kong SAR, China ; 4. National Engineering Research Center for Modernization of Traditional Chinese Medicine, Zhuhai 519020, China ; 5. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Belting 100094, China ; 6. College of Chemistry and Environmental Sciences, Hebei University, Baoding 071002, China |
| Abstract: | To investigate the correlation between genotype and distribution of Pogostemon cablin by sequencing ITS1 and ITS2 genes, and provide molecular information for its germplasm evaluation, ITS1 and ITS2 genes of Pogostemon cablin from different localities were identified by PCR direct sequencing. The sequences of ITS1 and ITS2 genes were 424 bp and 380 bp in length, respectively. And nineteen base substitutions were observed in ITS1 gene, and five in ITS2 gene. The results showed a good correlation between genotype and distribution of Pogostemon cablin, and ITS gene sequencing could provide useful molecular information for germplasm evaluation of the plant species verification. |
| Keywords: | Pogostemon cablin DNA sequencing germplasm evaluation |
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