龙胆药材中龙胆苦苷和马钱子苷酸含量的测定及其指纹图谱研究

龙胆（Radix gentianae）又名胆草，为龙胆科植物龙胆（Gentiana scabra Bge．）的根及根茎；性寒，味苦，可用于清热燥湿，泻肝胆火。其主要化学成分为裂环烯醚萜苷类化合物，三萜类化合物，多糖类化合物以及生物碱等。龙胆苦苷（C16H20O9）和马钱子苷酸（C16H24O10）是龙胆药材中含量较高的2种活性成分，属于裂环烯醚萜苷类化合物，2005版药典以龙胆苦苷为指标性成分对龙胆进行鉴别和含量测定。

Content of gentiopicroside and loganic acid in Radix gentianae and their fingerprints

To develop a HPLC-DAD-ESI-TOF/MS analysis method for the determination of gentiopicroside and loganic acid in Radix gentianae samples and for the research of their fingerprints. The samples were extracted using ASE for 10 min under 100 degrees C and 9.65 MPa, and divided into water phase and chloroform phase and analyzed them with HPLC-DAD-ESI-TOF/MS method respectively. Based on this method, the HPLC fingerprints of Radix gentianae were established. Comparing the spectrogram and mass spectrum of the chromatogram peak with the reference value, three compounds in water phase were identified as gentiopicroside, asafetida acid and loganic acid. There is no report of the compounds in chloroform phase. The content of gentiopicroside and loganic acid in samples of different groups were determined, separately. The fingerprints were compared by the software of the similarity evaluation system for chromatographic fingerprint. The water phase fingerprint congruence coefficients of samples from six different areas were above 0.90, however, the chloroform phase fingerprint congruence coefficients were within 0.62 -0.99. This method can be used for determination of potent component in Radix gentianae and its quality control. Radix gentianae from different producing areas have the largest diversities, and the diversities embodied in the content of chloroform phase compounds.