水杨柳根的化学成分

本文的目的是对水杨柳的根部进行化学成分研究， 采用硅胶柱色谱的方法分离和纯化化合物， 根据理化性质和波谱方法鉴定化合物结构。从水杨柳的根部分离得到了13个化合物， 包括1-羰基-油桐酸(1)， 油桐酸(2)， 3-乙酰氧基-油桐酸(3)， 蒲公英赛酮(4)， 蒲公英赛醇(5)， 3-乙酰氧基-12-齐墩果烯-28-酸甲酯(6)， 3-乙酰氧基-12-齐墩果烯-28-醇(7)， 熊果酸(8)， 羽扇豆醇(9)， 乙酰氧羽扇豆醇酯(10)， 臭矢菜素A(11)， 大黄酚(12)和没食子酸(13)。化合物1为新的蒲公英赛烷三萜类化合物， 化合物2～12均为首次从该植物中分离得到。并用MTT法测定了化合物1～3对AGZY 83-a和SMMC-7721细胞的抑制作用。证明化合物2对AGZY 83-a细胞有弱抑制作用(IC50 33.055 μg·mL^-1)。

Chemical constituents from the roots of Homonoia riparia

A new compound and twelve known compounds were isolated from the ethyl acetate extract of the roots of Homonoia riparia Lour, which are used in folk medicine for treatment of hepatitis, bellyache and scald, by the method of silica gel column chromatography repeatedly with a gradient of PE-EtOAc, PE-Me2CO, CHCl3-Me2CO, CHCl3-MeOH. Their structures were identified as a new compound 1-oxo-aleuritolic acid (1), and twelve known compounds aleuritolic acid (2), 3-acetoxy-aleuritolic acid (3), taraxerone (4), taraxerol (5), methyl 3-acetoxy-12-oleanen-28-oate (6), 3-acetoxy-12-oleanen-28-ol (7), ursolic acid (8), lupenol (9), 3beta-acetoxy-lupenol (10), cleomiscosin A (11), chrysophanol (12), and gallic acid (13), which were obtained from this plant for the first time, by the spectroscopic techniques of NMR, HMBC, IR and MS, separately. Among the cytotoxicities evaluation of compounds 1 -3 towards AGZY 83-a (human lung cancer cells) and SMMC-7721 (human liver cancer cells) tumor cells was assayed by MTT methods with cis-dichlorodiamminoplatinum (DDP) used as positive control. Compound 2 exerted weak activity against AGZY 83-a with the IC50 value of 33.055 microg x mL(-1), while 1 and 3 showed no activity to these two cell lines.