| 复合维生素B注射液中4种维生素含量测定 |
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| 引用本文： | 李祥胜,阚家义.复合维生素B注射液中4种维生素含量测定[J].安徽医药,2009,13(7):755-757. |
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| 作者姓名： | 李祥胜 阚家义 |
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| 作者单位： | 安徽省食品药品检验所,安徽,合肥,230051;安徽省食品药品检验所,安徽,合肥,230051 |
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| 摘 要： | 目的建立复合维生素B注射液中4种维生素的含量测定方法。方法采用高效液相色谱法，用甲醇和三乙胺-冰醋酸-甲醇-0．015mol·L^-1庚烷磺酸钠（0．2：7．5：75：820）为流动相，流速1．0ml·min^-1，检测波长为280nm。结果维生素B．、核黄素磷酸钠（以维生素B2计）、维生素B6、烟酰胺分别在1．6109-16．1091μg、0．1608-1．6075μg、0．1630-1．6298μg、2．4138-24．1379μg范围内呈良好线性关系，相关系数均达到0．999以上。平均回收率分别为100．7％（RSD％=0．27％，n=9）、101．1％（RSD％=0．73％，n=9）、100．5％（RSD％=0．38％，n=9）、100．6％（RSD％=1．04％，n=9）。结论本法准确、简便、快捷，可以同时测定复合维生素B注射液中维生素B1、核黄素磷酸钠（以维生素B1计）、维生素B6、烟酰胺的含量。
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| 关 键 词： | 复合维生素B注射液 维生素 高效液相色谱法 |
Assay of four vitamins in compoud vitamin B injection |
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| LI Xiang-sheng,KAN Jia-yi.Assay of four vitamins in compoud vitamin B injection[J].Anhui Medical and Pharmaceutical Journal,2009,13(7):755-757. |
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| Authors: | LI Xiang-sheng KAN Jia-yi |
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| Institution: | ( Anhui Institute for Drug Control, Hefei 230051 ) |
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| Abstract: | Aim To establish a method for assaying four vitamins in compound vitamin B injection.Methods By high performance liquid chromatography (HPLC) method,the mobile phase consisted of methanol and triethylamine-acetic acid-methanol-0.015 mol·L-1 sodium 1-heptanesulfonate (0.2∶ 7.5∶ 75∶ 820).The flow rate was 1.0 ml·min-1 and the detective wavelength was 280 nm.Results The method proved to be linear in the range of 1.6109～16.1091 μg,0.1608～1.6075 μg,0.1630～1.6298 μg,2.4138～24.1379 μg for VitB1,riboflavin sodium phosphate,VitB6 and nicotinamide,respectively.The correlation coefficients were all above 0.999.The average recovery rates were 100.7%(RSD=0.27%,n=9),101.1%(RSD=0.73%,n=9),100.5%(RSD=0.38%,n=9),100.6%(RSD=1.04%,n=9)respectively.Conclusion This method is accurate,simple and quick.It can assay VitB1,riboflavin sodium phosphate,VitB6 and nicotinamide in compound vitamin B injection simultaneously. |
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| Keywords: | compound vitamin B injection vitamins high performance liquid chromatography method |
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