A new human cell line derived from a poorly differentiated endometrial adenocarcinoma |
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| Authors: | David Gal J.Peter Forney V.G. Dev John C. Porter |
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| Affiliation: | Cecil H. and Ida Green Center for Reproductive Biology Sciences, Department of Obstetrics and Gynecology and the Division of Cytogenetics of the Department of Pathology, University of Texas Health Science Center, Southwestern Medical School, Dallas, Texas 75235 USA |
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| Abstract: | Establishment and characterization of an endometrial adenocarcinoma cell line are described. The tumor cells, designated AC-258, originated from a patient with a poorly differentiated adenocarcinoma of the endometrium. These cells have been passed in culture more than 102 times and have maintained their morphological and chromosomal integrity in a fashion suggestive of monocloning. Cells from passages 1, 58, and 102 were aneuploid. The average number of chromosomes per cell was 64.8, and 8–17 marker chromosomes per cell were identified and described. Histological evaluation of tumor explants from 3 passages grown in nude (athymic) mice revealed morphologic identity. The doubling time of AC-258 was 22 hr. No detectable estrogen- or progesterone-binding proteins were found. The AC-258 cell line provides researchers with another tool with which to investigate biochemical and biological properties of human cancer cells. |
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| Keywords: | To whom correspondence and requests for reprints should be addressed at: Department of Obstetrics and Gynecology University of Texas Health Science Center at Dallas 5323 Harry Hines Blvd. Dallas Tex. 75235. |
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