HPLC法测定不同产地黄蜀葵花中金丝桃苷含量

目的分析并比较了安徽四个不同产地黄蜀葵花药材中金丝桃苷的含量。方法高效液相色谱法，色谱柱Alltima C18（5μm，4．0mm×250mm），流动相：乙腈-水-磷酸（175：825：1），流速1．0ml·min^-1，检测波长：360nm，进样量：20μl，柱温为40℃。结果黄山黄蜀葵花中金丝桃苷含量1．18％，RSD为2．54％；合肥黄蜀葵花中金丝桃苷含量1．07％，RSD为1．62％；六安黄蜀葵花中金丝桃苷含量1．03％，RSD为1．94％；毫州黄蜀葵花中金丝桃苷含量0．92％，RSD为1.88％。结论HPLC法测得黄山黄蜀葵花中金丝桃苷含量最高，毫州黄蜀葵花中金丝桃苷含量最低，该方法简便准确，重现性好，为各地黄蜀葵花药材的进一步开发利用提供质量控制依据。

Determination of hyperine of flos abelmoschus manihot in four different areas of Anhui province

Aim To analyze and compared the content of hyperine in flos abelmoschus manihot of four different areas, Methods The column was Alltima C18 （5 μm,4.0 mm×250 mm）. The mobile phase was acetonitrile-water-phosphoric acid（175：825：1）. The flow rate was 1.0 ml·min^-1. The detection wavelength was 360 nm. The column temperature was 40℃. Results The content of hyperlne in the drug from Huangshan is 1.18% with RSD of 2.54%. The content of hyperine in the drug from Hefei is 1.07% with RSD of 1.62%. The content of Hyperine in the drug from Luan is 1.03% with RSD of 1.94%. The content of Hyperine in the drug from Bozhou is 0. 92% with RSD of 1.88%. Conclusion The content of Hyperine in the drug from Huangshan is the highest,while the content of Hyperine in the drug from Bozhou is the lowest. This method is simple,accurate and available for the exploitation and the quality control of flos abelmoschus manihot in different areas.