| 表达GST-NK4工程菌的发酵工艺研究 |
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| 引用本文： | 常冰梅,解军,王勇,张俊芳,张悦红,程牛亮,牛勃.表达GST-NK4工程菌的发酵工艺研究[J].中国生化药物杂志,2005,26(2):102-104. |
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| 作者姓名： | 常冰梅 解军 王勇 张俊芳 张悦红 程牛亮 牛勃 |
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| 作者单位： | 山西医科大学,生物化学与分子生物学教研室,山西,太原,030001 |
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| 摘 要： | 目的建立重组GST NK4融合基因工程菌的发酵工艺。方法采用摇瓶、发酵罐发酵,对影响工程菌生长和表达的条件如pH值、诱导时间及诱导剂浓度等进行优化。结果根据优化的条件,15L发酵罐发酵11h ,菌体收获量可达到湿重(2 4 .6±0 .98)g/L ,目的蛋白质的表达量占菌体总蛋白质的5 0 %左右。结论确定了周期短、产率高且稳定可靠的发酵工艺
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| 关 键 词： | NK4 大肠杆菌 发酵 |
| 文章编号： | 1005-1678(2005)02-0102-03 |
| 修稿时间： | 2004年4月12日 |
Study on the fermentation engineering of E.coli expressing GST-NK4 |
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| CHANG Bing-mei,XIE Jun,WANG Yong,ZHANG Jun-fang,ZHANG Yue-hong,CHENG Niu-liang,NIU Bo.Study on the fermentation engineering of E.coli expressing GST-NK4[J].Chinese Journal of Biochemical Pharmaceutics,2005,26(2):102-104. |
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| Authors: | CHANG Bing-mei XIE Jun WANG Yong ZHANG Jun-fang ZHANG Yue-hong CHENG Niu-liang NIU Bo |
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| Abstract: | PurposeTo develop the best fermentation procedure of E.colli expressing GST-NK4.MethodsOptimizing the the range of pH, induction time and concentration on the collection ratio and expression of the recombinant protein were analyzed with 15 liter fermentation tank.ResultsUnder the established conditions, (24.6±0.98)g/L of wet bacteria could be obtained. And the derivative of recombinant GST-NK4 was about 50% of total protein in the host.ConclusionThe established fermentative procedure increases the collection efficiency and expression of recombinant protein. |
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| Keywords: | recombinant NK4 E colli fermentation |
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