卵巢切除后脑缺血再灌海马CA1区ET-3的表达 |
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关 键 词: | 卵巢切除 脑缺血再灌海马 CA1区 ET-3 表达 |
Expression of ET-3 in hippocampal CA1 of ovariectomied mice after cerebral ischemia/reperfusion] |
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Authors: | Zhi-jun Zhang Zheng-qing Liu Zhi-jian Ma |
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Institution: | Heart and Brain Vascular Research Laboratory, Xiangya School of Medicine, Central South University, Changsha 410078, China. |
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Abstract: | OBJECTIVE: To detect the expression of endothelins-3 (ET-3) immunoactivity in hippocampal CA1 of ovariectomied (OVX) mice after ischemia/reperfusion (IR), and to further explore the relationship between estrogen and ET-3. METHODS: Fifty-four adult female mice were randomly divided into 3 groups. 1. IR group: the bilateral common carotid occlusion (BCCO) was carried out for 7 min on the 1st, 3rd, 5th and 14th day of reperfusion. 2. OVX/IR group: firstly the ovariectomy was performed, and after a week, BCCO was carried out for 7 min on the 1st, 3rd, 5th and 14th day of reperfusion (each time spot, n = 6). 3. sham-operated group (n = 6). RESULTS: Few ET-3 positive cells were diffusely distributed in the hippocampal CA1 region in the control group, whereas the expression of ET-3 increased in the experimental groups. The number of ET-3 positive cells significantly increased in the OVX/IR group compared with that of the IR group 1 d after the reperfusion (P < 0.01). It reached the maximum in both the OVX/IR and IR group on the 5th day of the reperfusion. CONCLUSION: Estrogen is involved in the regulation of ET-3 in the early stage of the reperfusion, and probably plays a protective role in the survival of neurons. |
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