| Expression of Cystic Fibrosis Transmembrane Conductance Regulator in Rat Ovary |
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| 作者姓名： | 靳镭 汤瑞玲 |
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| 作者单位： | Department of Obstetrics and Gynecology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology |
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| 基金项目： | Acknowledgement The authors are grateful to Professor Mulan Yang for technical assistance. |
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| 摘 要： | The protein expression of cystic fibrosis transmembrane conductance regulator （CFTR）, a cAMP-activated Cl- channel, in ovarian stimulated premature female rat ovary during a cycle of follicle development and corpus luteum formation was investigated. Animals were injected with 10 U pregnant Mare＇s serum gonadotropin （PMSG） and subsequently 10 U hCG 48 h later. Time-dependent immunohistochemistry and Western blotting experiments were performed before and 24, 48, 72 h after hCG treatment. The immunohistochemistry revealed that administration of PMSG stimulated the CFTR expression in thecal cell layer and granulosa cell layer of mature follicles 48 h post injection, coincident with the PMSG-induced peak in follicular estradiol. However, the expression of CFTR in the granulose lutein cell layer and thecal lutein cell layer was time-dependently reduced following hCG injection, in accordance with the gradually increased progestogen level during luteum corpus formation. Western blotting analysis demonstrated that rat ovarian tissue expressed the special CFTR band at 170 kD. It is concluded that cAMP-dependent Cl- channels are involved in regulation of follicle development and luteum formation.
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| 关 键 词： | 囊性纤维化 雌性大鼠 卵巢刺激 调节器 孕马血清促性腺激素 跨膜传导 时间依赖性 CFTR |
Expression of cystic fibrosis transmembrane conductance regulator in rat ovary |
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| Lei Jin,Ruiling Tang.Expression of Cystic Fibrosis Transmembrane Conductance Regulator in Rat Ovary[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2008,28(5):584-587. |
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| Authors: | Lei Jin Ruiling Tang |
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| Institution: | Reproductive Medical Center, Department of Obstetrics and Gynecology, Tong/i Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | The protein expression of cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-activated Cl(-) channel, in ovarian stimulated premature female rat ovary during a cycle of follicle development and corpus luteum formation was investigated. Animals were injected with 10 U pregnant Mare's serum gonadotropin (PMSG) and subsequently 10 U hCG 48 h later. Time-dependent immunohistochemistry and Western blotting experiments were performed before and 24, 48, 72 h after hCG treatment. The immunohistochemistry revealed that administration of PMSG stimulated the CFTR expression in thecal cell layer and granulosa cell layer of mature follicles 48 h post injection, coincident with the PMSG-induced peak in follicular estradiol. However, the expression of CFTR in the granulose lutein cell layer and thecal lutein cell layer was time-dependently reduced following hCG injection, in accordance with the gradually increased progestogen level during luteum corpus formation. Western blotting analysis demonstrated that rat ovarian tissue expressed the special CFTR band at 170 kD. It is concluded that cAMP-dependent Cl(-) channels are involved in regulation of follicle development and luteum formation. |
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| Keywords: | cystic fibrosis transmembrane conductance regulator Cl- channel rat ovary follicle corpus luteum |
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